Editing of an alpha-kafirin gene family increases digestibility and protein quality in sorghum

Aixia Li, Shangang Jia, Abou Yobi, Zhengxiang Ge, Shirley J. Sato, Chi Zhang, Ruthie Angelovici, Thomas E. Clemente, David R. Holding

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Kafirins are the major storage proteins in sorghum (Sorghum bicolor) grains and form protein bodies with poor digestibility. Since kafirins are devoid of the essential amino acid lysine, they also impart poor protein quality to the kernel. The α-kafirins, which make up most of the total kafirins, are largely encoded by the k1C family of highly similar genes. We used a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing approach to target the k1C genes to create variants with reduced kafirin levels and improved protein quality and digestibility. A single guide RNA was designed to introduce mutations in a conserved region encoding the endoplasmic reticulum signal peptide of α-kafirins. Sequencing of kafirin PCR products revealed extensive edits in 25 of 26 events in one or multiple k1C family members. T1 and T2 seeds showed reduced α-kafirin levels, and selected T2 events showed significantly increased grain protein digestibility and lysine content. Thus, a single consensus single guide RNA carrying target sequence mismatches is sufficient for extensive editing of all k1C genes. The resulting quality improvements can be deployed rapidly for breeding and the generation of transgene-free, improved cultivars of sorghum, a major crop worldwide.

Original languageEnglish (US)
Pages (from-to)1425-1438
Number of pages14
JournalPlant Physiology
Volume177
Issue number4
DOIs
StatePublished - Aug 1 2018

Fingerprint

kafirins
Sorghum
Sorghum (Poaceae)
digestibility
Clustered Regularly Interspaced Short Palindromic Repeats
Guide RNA
Genes
Proteins
genes
proteins
Lysine
lysine
Essential Amino Acids
RNA
Protein Sorting Signals
Quality Improvement
Transgenes
Endoplasmic Reticulum
protein bodies
Breeding

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Editing of an alpha-kafirin gene family increases digestibility and protein quality in sorghum. / Li, Aixia; Jia, Shangang; Yobi, Abou; Ge, Zhengxiang; Sato, Shirley J.; Zhang, Chi; Angelovici, Ruthie; Clemente, Thomas E.; Holding, David R.

In: Plant Physiology, Vol. 177, No. 4, 01.08.2018, p. 1425-1438.

Research output: Contribution to journalArticle

Li, A, Jia, S, Yobi, A, Ge, Z, Sato, SJ, Zhang, C, Angelovici, R, Clemente, TE & Holding, DR 2018, 'Editing of an alpha-kafirin gene family increases digestibility and protein quality in sorghum', Plant Physiology, vol. 177, no. 4, pp. 1425-1438. https://doi.org/10.1104/pp.18.00200
Li, Aixia ; Jia, Shangang ; Yobi, Abou ; Ge, Zhengxiang ; Sato, Shirley J. ; Zhang, Chi ; Angelovici, Ruthie ; Clemente, Thomas E. ; Holding, David R. / Editing of an alpha-kafirin gene family increases digestibility and protein quality in sorghum. In: Plant Physiology. 2018 ; Vol. 177, No. 4. pp. 1425-1438.
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