Ecotoxicological risks associated with land treatment of petrochemical wastes. II. Effects on hepatic phase I and phase II detoxification enzymes in cotton rats

Ruth I. Carlson, James A. Wilson, Robert L. Lochmiller, David M. Janz, Jackie L. Schroder, Nicholas T. Basta

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The purpose of this study was to evaluate possible exposure and resultant hepatic effects of petrochemical waste on cotton rats (Sigmodon hispidus) living on landfarmed sites. Male and female cotton rats were collected in summer, fall, and winter from four landfarm sites and four ecologically similar reference sites. Hepatic methoxyresorufin O-deethylase (MROD) activity was significantly induced in male and female rats collected from landfarms compared to rats collected from reference sites. In contrast, changes in ethoxyresorufin O-deethylase (EROD) activity were inconsistent due to season, sex, and treatment variation. A significant decrease in EROD and MROD activity was found in cotton rats held for 48 h prior to sacrifice compared to rats euthanized on the day of capture. These results indicate that when using hepatic EROD and MROD activities as biochemical markers of exposure to aryl hydrocarbon receptor agonists, animals should be euthanized as quickly as possible after capture. The cotton rats collected from one landfarm unit exhibited a pattern of consistent elevation of EROD, MROD, and pentoxyresorufin O-deethylase (PROD) activity. This unit also had a pattern of elevated CYP1A2 protein expression determined by Western blotting. There were no consistent alterations from contaminant exposure on hepatic glutathione S-transferase (GST) activity, glutathione levels, or CYP1A1 protein. Hepatic EROD and MROD activities varied considerably between seasons and sex of rats. In conclusion, consistent induction of hepatic EROD and MROD activities in cotton rats was found in three out of four sampled landfarm sites compared to the rats collected from the reference sites, indicating exposure to contaminants - likely polyaromatic hydrocarbons.

Original languageEnglish (US)
Pages (from-to)327-343
Number of pages17
JournalJournal of Toxicology and Environmental Health - Part A
Volume66
Issue number4
DOIs
StatePublished - Feb 28 2003
Externally publishedYes

Fingerprint

Phase II Metabolic Detoxication
Sigmodontinae
Detoxification
Cytochrome P-450 CYP1A1
detoxification
Petrochemicals
Cotton
cotton
Rats
Enzymes
enzyme
Liver
hydrocarbon
protein
pollutant
Aryl Hydrocarbon Receptors
Cytochrome P-450 CYP1A2
Hydrocarbons
petrochemical
land

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Ecotoxicological risks associated with land treatment of petrochemical wastes. II. Effects on hepatic phase I and phase II detoxification enzymes in cotton rats. / Carlson, Ruth I.; Wilson, James A.; Lochmiller, Robert L.; Janz, David M.; Schroder, Jackie L.; Basta, Nicholas T.

In: Journal of Toxicology and Environmental Health - Part A, Vol. 66, No. 4, 28.02.2003, p. 327-343.

Research output: Contribution to journalArticle

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