Ecometagenetics confirm high tropical rainforest nematode diversity

Dorota L. Porazinska, Robin M. Giblin-Davis, Alejandro Esquivel, Thomas O. Powers, Way Sung, W. Kelley Thomas

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The general patterns of increasing biodiversity from the poles to the equator have been well documented for large terrestrial organisms such as plants and vertebrates but are largely unknown for microbiota. In contrast to macrobiota, microbiota have long been assumed to exhibit cosmopolitan, random distributions and a lack of spatial patterns. To evaluate the assumption, we conducted a survey of nematode diversity within the soil, litter and canopy habitats of the humid lowland tropical rainforest of Costa Rica using an ultrasequencing ecometagenetic approach at a species-equivalent taxonomic level. Our data indicate that both richness and diversity of nematode communities in the tropical rainforests of Costa Rica are high and exceed observed values from temperate ecosystems. The majority of nematode species were unknown to science, providing evidence for the presence of highly endemic (not cosmopolitan) species of still completely undiscovered biodiversity. Most importantly, the greater taxonomic resolution used here allowed us to reveal predictable habitat associations for specific taxa and thus gain insights into their nonrandom distribution patterns.

Original languageEnglish (US)
Pages (from-to)5521-5530
Number of pages10
JournalMolecular Ecology
Volume19
Issue number24
DOIs
StatePublished - Dec 1 2010

Fingerprint

tropical rain forests
rainforest
Ecosystem
nematode
Costa Rica
Microbiota
Biodiversity
Nematoda
biodiversity
habitat
habitats
Vertebrates
lowlands
vertebrate
litter
Soil
vertebrates
canopy
ecosystems
organisms

Keywords

  • DNA barcoding
  • biodiversity
  • ecometagenetic
  • nematode
  • next generation sequencing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Porazinska, D. L., Giblin-Davis, R. M., Esquivel, A., Powers, T. O., Sung, W., & Thomas, W. K. (2010). Ecometagenetics confirm high tropical rainforest nematode diversity. Molecular Ecology, 19(24), 5521-5530. https://doi.org/10.1111/j.1365-294X.2010.04891.x

Ecometagenetics confirm high tropical rainforest nematode diversity. / Porazinska, Dorota L.; Giblin-Davis, Robin M.; Esquivel, Alejandro; Powers, Thomas O.; Sung, Way; Thomas, W. Kelley.

In: Molecular Ecology, Vol. 19, No. 24, 01.12.2010, p. 5521-5530.

Research output: Contribution to journalArticle

Porazinska, DL, Giblin-Davis, RM, Esquivel, A, Powers, TO, Sung, W & Thomas, WK 2010, 'Ecometagenetics confirm high tropical rainforest nematode diversity', Molecular Ecology, vol. 19, no. 24, pp. 5521-5530. https://doi.org/10.1111/j.1365-294X.2010.04891.x
Porazinska DL, Giblin-Davis RM, Esquivel A, Powers TO, Sung W, Thomas WK. Ecometagenetics confirm high tropical rainforest nematode diversity. Molecular Ecology. 2010 Dec 1;19(24):5521-5530. https://doi.org/10.1111/j.1365-294X.2010.04891.x
Porazinska, Dorota L. ; Giblin-Davis, Robin M. ; Esquivel, Alejandro ; Powers, Thomas O. ; Sung, Way ; Thomas, W. Kelley. / Ecometagenetics confirm high tropical rainforest nematode diversity. In: Molecular Ecology. 2010 ; Vol. 19, No. 24. pp. 5521-5530.
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