Echium oil reduces plasma triglycerides by increasing intravascular lipolysis in apob100-only low density lipoprotein (LDL) receptor knockout mice

Lolita M. Forrest, Christopher M. Lough, Soonkyu Chung, Elena Y. Boudyguina, Abraham K. Gebre, Thomas L. Smith, Perry L. Colvin, John S. Parks

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Echium oil (EO), which is enriched in SDA (18:4 n-3), reduces plasma triglyceride (TG) concentrations in humans and mice. We compared mechanisms by which EO and fish oil (FO) reduce plasma TG concentrations in mildly hypertriglyceridemic male apoB100-only LDLrKO mice. Mice were fed one of three atherogenic diets containing 0.2% cholesterol and palm oil (PO; 20%), EO (10% EO + 10% PO), or FO (10% FO + 10% PO). Livers from PO- and EO-fed mice had similar TG and cholesteryl ester (CE) content, which was significantly higher than in FO-fed mice. Plasma TG secretion was reduced in FO vs. EO-fed mice. Plasma very low density lipoprotein (VLDL) particle size was ordered: PO (63 ± 4 nm) > EO (55 ± 3 nm) > FO (40 ± 2 nm). Post-heparin lipolytic activity was similar among groups, but TG hydrolysis by purified lipoprotein lipase was significantly greater for EO and FO VLDL compared to PO VLDL. Removal of VLDL tracer from plasma was marginally faster in EO vs. PO fed mice. Our results suggest that EO reduces plasma TG primarily through increased intravascular lipolysis of TG and VLDL clearance. Finally, EO may substitute for FO to reduce plasma TG concentrations, but not hepatic steatosis in this mouse model.

Original languageEnglish (US)
Pages (from-to)2629-2645
Number of pages17
JournalNutrients
Volume5
Issue number7
DOIs
StatePublished - Jul 12 2013

Fingerprint

Echium
LDL Receptors
Lipolysis
lipolysis
low density lipoprotein
Knockout Mice
Fish Oils
Oils
Triglycerides
triacylglycerols
fish oils
oils
receptors
mice
very low density lipoprotein
VLDL Lipoproteins
Atherogenic Diet
lipoprotein lipase
cholesteryl esters
Lipoprotein Lipase

Keywords

  • Botanical oil
  • Fish oil
  • Hepatic steatosis
  • N-3 fatty acids
  • Omega-3 fatty acids
  • Polyunsaturated fatty acids
  • Pufa
  • Very low density lipoproteins

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Echium oil reduces plasma triglycerides by increasing intravascular lipolysis in apob100-only low density lipoprotein (LDL) receptor knockout mice. / Forrest, Lolita M.; Lough, Christopher M.; Chung, Soonkyu; Boudyguina, Elena Y.; Gebre, Abraham K.; Smith, Thomas L.; Colvin, Perry L.; Parks, John S.

In: Nutrients, Vol. 5, No. 7, 12.07.2013, p. 2629-2645.

Research output: Contribution to journalArticle

Forrest, Lolita M. ; Lough, Christopher M. ; Chung, Soonkyu ; Boudyguina, Elena Y. ; Gebre, Abraham K. ; Smith, Thomas L. ; Colvin, Perry L. ; Parks, John S. / Echium oil reduces plasma triglycerides by increasing intravascular lipolysis in apob100-only low density lipoprotein (LDL) receptor knockout mice. In: Nutrients. 2013 ; Vol. 5, No. 7. pp. 2629-2645.
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