Ebolavirus VP24 binding to karyopherins is required for inhibition of interferon signaling

Mathieu Mateo, St Patrick Reid, Lawrence W. Leung, Christopher F. Basler, Viktor E. Volchkov

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The Ebolavirus VP24 protein counteracts alpha/beta interferon (IFN-α/β) and IFN-γ signaling by blocking the nuclear accumulation of tyrosine-phosphorylated STAT1 (PY-STAT1). According to the proposed model, VP24 binding to members of the NPI-1 subfamily of karyopherin alpha (KPNα) nuclear localization signal receptors prevents their binding to PY-STAT1, thereby preventing PY-STAT1 nuclear accumulation. This study now identifies two domains of VP24 required for inhibition of IFN-β-induced gene expression and PY-STAT1 nuclear accumulation. We demonstrate that loss of function correlates with loss of binding to KPNα proteins. Thus, the VP24 IFN antagonist function requires the ability of VP24 to interact with KPNα.

Original languageEnglish (US)
Pages (from-to)1169-1175
Number of pages7
JournalJournal of virology
Volume84
Issue number2
DOIs
StatePublished - Jan 1 2010

Fingerprint

karyopherins
Ebolavirus
alpha Karyopherins
Karyopherins
interferons
Interferons
interferon-beta
Nuclear Localization Signals
nuclear localization signals
Interferon-beta
Interferon-alpha
Tyrosine
tyrosine
antagonists
Proteins
proteins
Gene Expression
gene expression
receptors

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Ebolavirus VP24 binding to karyopherins is required for inhibition of interferon signaling. / Mateo, Mathieu; Reid, St Patrick; Leung, Lawrence W.; Basler, Christopher F.; Volchkov, Viktor E.

In: Journal of virology, Vol. 84, No. 2, 01.01.2010, p. 1169-1175.

Research output: Contribution to journalArticle

Mateo, Mathieu ; Reid, St Patrick ; Leung, Lawrence W. ; Basler, Christopher F. ; Volchkov, Viktor E. / Ebolavirus VP24 binding to karyopherins is required for inhibition of interferon signaling. In: Journal of virology. 2010 ; Vol. 84, No. 2. pp. 1169-1175.
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