Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2α includes cytokine signaling

Heather Talbott, Xiaoying Hou, Fang Qiu, Pan Zhang, Chittibabu Guda, Fang Yu, Robert A. Cushman, Jennifer R Wood, Cheng Wang, Andrea S Cupp, John S Davis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5–4 h) was performed on cows at midcycle. A microarray-determined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2α effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-κB and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of temporal signaling cascades. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly altered by PGF2α treatment, which both, directly and indirectly, indicated cytokine activation. Elevated levels of cytokine transcripts after PGF2α and predicted activation of cytokine pathways implicate inflammatory reactions early in PGF2α-mediated luteolysis.

Original languageEnglish (US)
Pages (from-to)93-109
Number of pages17
JournalMolecular and Cellular Endocrinology
Volume452
DOIs
StatePublished - Sep 5 2017

Fingerprint

Dinoprost
Corpus Luteum
Transcriptome
Cytokines
Luteolysis
Chemical activation
Estrous Cycle
Self organizing maps
Ruminants
Bioinformatics
Microarrays
Computational Biology
Fertility
Genes
Messenger RNA

Keywords

  • Corpus luteum
  • Cytokine
  • Luteolysis
  • PGF2α
  • Regression
  • Signaling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2α includes cytokine signaling. / Talbott, Heather; Hou, Xiaoying; Qiu, Fang; Zhang, Pan; Guda, Chittibabu; Yu, Fang; Cushman, Robert A.; Wood, Jennifer R; Wang, Cheng; Cupp, Andrea S; Davis, John S.

In: Molecular and Cellular Endocrinology, Vol. 452, 05.09.2017, p. 93-109.

Research output: Contribution to journalArticle

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