Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation

Quanlong Lu, Christine Insinna, Carolyn Ott, Jimmy Stauffer, Petra A. Pintado, Juliati Rahajeng, Ulrich Baxa, Vijay Walia, Adrian Cuenca, Yoo Seok Hwang, Ira O. Daar, Susana Lopes, Jennifer Lippincott-Schwartz, Peter K. Jackson, Steven H Caplan, Christopher J. Westlake

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Membrane association with mother centriole (M-centriole) distal appendages is critical for ciliogenesis initiation. How the Rab GTPase Rab11-Rab8 cascade functions in early ciliary membrane assembly is unknown. Here, we show that the membrane shaping proteins EHD1 and EHD3, in association with the Rab11-Rab8 cascade, function in early ciliogenesis. EHD1 and EHD3 localize to preciliary membranes and the ciliary pocket. EHD-dependent membrane tubulation is essential for ciliary vesicle formation from smaller distal appendage vesicles (DAVs). Importantly, this step functions in M-centriole to basal body transformation and recruitment of transition zone proteins and IFT20. SNAP29, a SNARE membrane fusion regulator and EHD1-binding protein, is also required for DAV-mediated ciliary vesicle assembly. Interestingly, only after ciliary vesicle assembly is Rab8 activated for ciliary growth. Our studies uncover molecular mechanisms informing a previously uncharacterized ciliogenesis step, whereby EHD1 and EHD3 reorganize the M-centriole and associated DAVs before coordinated ciliary membrane and axoneme growth.

Original languageEnglish (US)
Pages (from-to)228-240
Number of pages13
JournalNature Cell Biology
Volume17
Issue number3
DOIs
StatePublished - Mar 2 2015

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Cilia
Centrioles
Membranes
rab GTP-Binding Proteins
Basal Bodies
Axoneme
SNARE Proteins
Membrane Fusion
Growth
Carrier Proteins
Membrane Proteins
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lu, Q., Insinna, C., Ott, C., Stauffer, J., Pintado, P. A., Rahajeng, J., ... Westlake, C. J. (2015). Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Nature Cell Biology, 17(3), 228-240. https://doi.org/10.1038/ncb3109

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. / Lu, Quanlong; Insinna, Christine; Ott, Carolyn; Stauffer, Jimmy; Pintado, Petra A.; Rahajeng, Juliati; Baxa, Ulrich; Walia, Vijay; Cuenca, Adrian; Hwang, Yoo Seok; Daar, Ira O.; Lopes, Susana; Lippincott-Schwartz, Jennifer; Jackson, Peter K.; Caplan, Steven H; Westlake, Christopher J.

In: Nature Cell Biology, Vol. 17, No. 3, 02.03.2015, p. 228-240.

Research output: Contribution to journalArticle

Lu, Q, Insinna, C, Ott, C, Stauffer, J, Pintado, PA, Rahajeng, J, Baxa, U, Walia, V, Cuenca, A, Hwang, YS, Daar, IO, Lopes, S, Lippincott-Schwartz, J, Jackson, PK, Caplan, SH & Westlake, CJ 2015, 'Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation', Nature Cell Biology, vol. 17, no. 3, pp. 228-240. https://doi.org/10.1038/ncb3109
Lu Q, Insinna C, Ott C, Stauffer J, Pintado PA, Rahajeng J et al. Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Nature Cell Biology. 2015 Mar 2;17(3):228-240. https://doi.org/10.1038/ncb3109
Lu, Quanlong ; Insinna, Christine ; Ott, Carolyn ; Stauffer, Jimmy ; Pintado, Petra A. ; Rahajeng, Juliati ; Baxa, Ulrich ; Walia, Vijay ; Cuenca, Adrian ; Hwang, Yoo Seok ; Daar, Ira O. ; Lopes, Susana ; Lippincott-Schwartz, Jennifer ; Jackson, Peter K. ; Caplan, Steven H ; Westlake, Christopher J. / Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. In: Nature Cell Biology. 2015 ; Vol. 17, No. 3. pp. 228-240.
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