Early childhood infection of Kaposi's sarcoma-associated herpesvirus in Zambian households

A molecular analysis

Landon N. Olp, Danielle M. Shea, Maxine K. White, Clement Gondwe, Chipepo Kankasa, Charles Wood

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Sub-Saharan Africa is endemic for Kaposi's sarcoma-associated herpesvirus (KSHV) and there is a high rate of early childhood infection; however, the transmission sources are not well characterized. We examined household members as potential KSHV transmission sources to young children in the KSHV-endemic country of Zambia. To this end, we enrolled and followed Zambian households with at least one KSHV-seropositive child and collected longitudinal buccal swab samples. KSHV burden was evaluated and K1 sequences from the children were determined and analyzed for differences to K1 sequences from household members. The K1 sequences were also analyzed for evolution over time. We generated K1 sequences from 31 individuals across 16 households. Nine households contained multiple KSHV-positive members, including at least one child. In six out of the nine households, the child had 100% sequence identity to all household members. However, in two households the child and mother had distinct K1 sequences. In the remaining household, the children were the only KSHV-infected individuals. Furthermore, we report that 1 out of 18 individuals had K1 sequence variation within the timespan analyzed. In our study, we provide evidence that (i) early childhood KSHV transmission occurs from both within and outside the household, (ii) intrahousehold transmission can occur via nonmaternal sources, (iii) viral shedding in the buccal cavity is highly variable and (iv) the dominant K1 sequence within an individual did not rapidly evolve over time. These results are important for developing KSHV intervention strategies.

Original languageEnglish (US)
Pages (from-to)1182-1190
Number of pages9
JournalInternational Journal of Cancer
Volume132
Issue number5
DOIs
StatePublished - Mar 1 2013

Fingerprint

Human Herpesvirus 8
Infection
Cheek
Virus Shedding
Zambia
Africa South of the Sahara
Mothers

Keywords

  • K1
  • Kaposi's sarcoma-associated herpesvirus
  • Zambia
  • children
  • households
  • transmission

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Early childhood infection of Kaposi's sarcoma-associated herpesvirus in Zambian households : A molecular analysis. / Olp, Landon N.; Shea, Danielle M.; White, Maxine K.; Gondwe, Clement; Kankasa, Chipepo; Wood, Charles.

In: International Journal of Cancer, Vol. 132, No. 5, 01.03.2013, p. 1182-1190.

Research output: Contribution to journalArticle

Olp, Landon N. ; Shea, Danielle M. ; White, Maxine K. ; Gondwe, Clement ; Kankasa, Chipepo ; Wood, Charles. / Early childhood infection of Kaposi's sarcoma-associated herpesvirus in Zambian households : A molecular analysis. In: International Journal of Cancer. 2013 ; Vol. 132, No. 5. pp. 1182-1190.
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