Dysfunction of immune system and induction of autoantibodies to liver antigens by neonatal thymectomy in mice

Mitsuru Myozaki, Mei Qi Wang, Yasuhiro Yoshida, Jia Nuo Liu, Etsushi Kuroda, Uki Yamashita

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3 Scopus citations

Abstract

We examined the development of autoantibodies to liver proteins and hepatitis in BALB/c mice thymectomized 2 days after birth and attempted to characterize the immune function of these mice. Autoantibodies to crude liver proteins detected by ELISA were found in 21(84%) of 25 mice thymectomized 2 days after birth. In these mice, sera of 11 animals showed reactivity with both liver specific proteins (LSP) and the second fraction of crude liver proteins; sera of 3 mice showed reactivity with only the second fraction but no sera showed reactivity with only LSP. By Western-immunoblotting, sera of BALB/c mice which showed high autoantibody level to liver proteins detected a strong band around 150kD in the second fraction of crude liver proteins. Still more, hepatic inflammation; mononuclear cell infiltration in the portal area, was induced in mice with apparently high autoantibody level to crude liver proteins. These results in BALB/c mice corresponded with our previous reports which employed C3H/HeN mice. Next, we examined immune functions of mice thymectomized 2 days after birth. In thymectomized mice, the proportion of Thy-1, L3T4 and Lyt-2 positive cells (T cells) decreased and the proportion of B220 positive cells (B cells) increased. The proliferative response of lymph node lymphocytes cultured with mitomycin C-treated syngeneic spleen cells was lower, and the total IgG level in the sera was higher when compared with control normal mice. Anti-nuclear antibody (ANA) also appeared in the sera of thymectomized mice 2 days after birth. All these results suggest that the dysfunction of T cell and polyclonal activation of B cell were induced in neonatally thymectomized mice and resulted in the production of ANA and autoantibodies to liver proteins.

Original languageEnglish (US)
Pages (from-to)75-83
Number of pages9
JournalHiroshima Journal of Medical Sciences
Volume45
Issue number3
Publication statusPublished - Sep 1 1996

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Keywords

  • Autoantibody to liver
  • Immune dysfunction
  • Neonatal thymectomy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Myozaki, M., Wang, M. Q., Yoshida, Y., Liu, J. N., Kuroda, E., & Yamashita, U. (1996). Dysfunction of immune system and induction of autoantibodies to liver antigens by neonatal thymectomy in mice. Hiroshima Journal of Medical Sciences, 45(3), 75-83.