Dynamics of the Interaction of RecG Protein with Stalled Replication Forks

Zhiqiang Sun, Mohtadin Hashemi, Galina Warren, Piero R. Bianco, Yuri L Lyubchenko

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

As a guardian of the bacterial genome, the RecG DNA helicase repairs DNA replication and rescues stalled replication. We applied atomic force microscopy (AFM) to directly visualize dynamics of RecG upon the interaction with replication fork substrates in the presence and absence of SSB using high-speed AFM. We directly visualized that RecG moves back and forth over dozens of base pairs in the presence of SSB. There is no RecG translocation in the absence of SSB. Computational modeling was performed to build models of Escherichia coli RecG in a free state and in complex with the fork. The simulations revealed the formation of complexes of RecG with the fork and identified conformational transitions that may be responsible for RecG remodeling that can facilitate RecG translocation along the DNA duplex. Such complexes do not form with the DNA duplex, which is in line with experimental data. Overall, our results provide mechanistic insights into the modes of interaction of RecG with the replication fork, suggesting a novel role of RecG in the repair of stalled DNA replication forks.

Original languageEnglish (US)
Pages (from-to)1967-1976
Number of pages10
JournalBiochemistry
Volume57
Issue number13
DOIs
StatePublished - Apr 3 2018

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Atomic Force Microscopy
DNA Replication
DNA Helicases
Bacterial Genomes
DNA
Base Pairing
DNA Repair
Atomic force microscopy
Proteins
Repair
Escherichia coli
Genes
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dynamics of the Interaction of RecG Protein with Stalled Replication Forks. / Sun, Zhiqiang; Hashemi, Mohtadin; Warren, Galina; Bianco, Piero R.; Lyubchenko, Yuri L.

In: Biochemistry, Vol. 57, No. 13, 03.04.2018, p. 1967-1976.

Research output: Contribution to journalArticle

Sun, Zhiqiang ; Hashemi, Mohtadin ; Warren, Galina ; Bianco, Piero R. ; Lyubchenko, Yuri L. / Dynamics of the Interaction of RecG Protein with Stalled Replication Forks. In: Biochemistry. 2018 ; Vol. 57, No. 13. pp. 1967-1976.
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