Dynamics of dendritic spines and their afferent terminals

Spines are more motile than presynaptic boutons

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Previous work has established that dendritic spines, sites of excitatory input in CNS neurons, can be highly dynamic, in later development as well as in mature brain. Although spine motility has been proposed to facilitate the formation of new synaptic contacts, we have reported that spines continue to be dynamic even if they bear synaptic contacts. An outstanding question related to this finding is whether the presynaptic terminals that contact dendritic spines are as dynamic as their postsynaptic targets. Using multiphoton time-lapse microscopy of GFP-labeled Purkinje cells and DiI-labeled granule cell parallel fiber afferents in cerebellar slices, we monitored the dynamic behavior of both presynaptic terminals and postsynaptic dendritic spines in the same preparation. We report that while spines are dynamic, the presynaptic terminals they contact are quite stable. We confirmed the relatively low levels of presynaptic terminal motility by imaging parallel fibers in vivo. Finally, spine motility can occur when a functional presynaptic terminal is apposed to it. These analyses further call into question the function of spine motility, and to what extent the synapse breaks or maintains its contact during the movement of the spine.

Original languageEnglish (US)
Pages (from-to)366-377
Number of pages12
JournalDevelopmental Biology
Volume277
Issue number2
DOIs
StatePublished - Jan 15 2005

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Dendritic Spines
Presynaptic Terminals
Spine
Purkinje Cells
Synapses
Microscopy
Neurons
Brain

Keywords

  • Cerebellum
  • Dendritic spines
  • FM1-43
  • Synaptic terminals
  • Synaptogenesis
  • Two-photon

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Dynamics of dendritic spines and their afferent terminals : Spines are more motile than presynaptic boutons. / Deng, Jinbo; Dunaevsky-Hutt, Anna.

In: Developmental Biology, Vol. 277, No. 2, 15.01.2005, p. 366-377.

Research output: Contribution to journalArticle

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