Dynamic subunit stoichiometry confers a progressive continuum of pharmacological sensitivity by KCNQ potassium channels

Haibo Yu, Zhihong Lin, Margrith E. Mattmann, Beiyan Zou, Cecile Terrenoire, Hongkang Zhang, Meng Wu, Owen B. McManus, Robert S. Kass, Craig W. Lindsley, Corey R. Hopkins, Min Li

Research output: Contribution to journalArticle

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Abstract

Voltage-gated KCNQ1 (Kv7.1) potassium channels are expressed abundantly in heart but they are also found in multiple other tissues. Differential coassembly with single transmembrane KCNE beta subunits in different cell types gives rise to a variety of biophysical properties, hence endowing distinct physiological roles for KCNQ1- KCNEx complexes. Mutations in either KCNQ1 or KCNE1 genes result in diseases in brain, heart, and the respiratory system. In addition to complexities arising from existence of five KCNE subunits, KCNE1 to KCNE5, recent studies in heterologous systems suggest unorthodox stoichiometric dynamics in subunit assembly is dependent on KCNE expression levels. The resultant KCNQ1-KCNE channel complexes may have a range of zero to two or even up to four KCNE subunits coassembling per KCNQ1 tetramer. These findings underscore the need to assess the selectivity of small-molecule KCNQ1 modulators on these different assemblies. Herewe report a unique small-molecule gating modulator, ML277, that potentiates both homomultimeric KCNQ1 channels and unsaturated heteromultimeric (KCNQ1)4(KCNE1)n (n < 4) channels. Progressive increase of KCNE1 or KCNE3 expression reduces efficacy of ML277 and eventually abolishes ML277-mediated augmentation. In cardiomyocytes, the slowly activating delayed rectifier potassium current, or IKs, is believed to be a heteromultimeric combination of KCNQ1 and KCNE1, but it is not entirely clear whether IKs is mediated by KCNE-saturated KCNQ1 channels or by channels with intermediate stoichiometries. We found ML277 effectively augments IKs current of cultured human cardiomyocytes and shortens action potential duration. These data indicate that unsaturated heteromultimeric (KCNQ1)4(KCNE1)n channels are present as components of IKs and are pharmacologically distinct from KCNE-saturated KCNQ1- KCNE1 channels.

Original languageEnglish (US)
Pages (from-to)8732-8737
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number21
DOIs
StatePublished - May 21 2013

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KCNQ Potassium Channels
KCNQ1 Potassium Channel
Pharmacology
Cardiac Myocytes
Voltage-Gated Potassium Channels
Brain Diseases
Respiratory System
Action Potentials
Potassium
Mutation
(R)-N-(4-(4-methoxyphenyl)thiazol-2-yl)-1-tosylpiperidine-2-carboxamide
Genes

Keywords

  • Cardiac physiology
  • Drug
  • Long QT
  • Pharmacology

ASJC Scopus subject areas

  • General

Cite this

Dynamic subunit stoichiometry confers a progressive continuum of pharmacological sensitivity by KCNQ potassium channels. / Yu, Haibo; Lin, Zhihong; Mattmann, Margrith E.; Zou, Beiyan; Terrenoire, Cecile; Zhang, Hongkang; Wu, Meng; McManus, Owen B.; Kass, Robert S.; Lindsley, Craig W.; Hopkins, Corey R.; Li, Min.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 21, 21.05.2013, p. 8732-8737.

Research output: Contribution to journalArticle

Yu, Haibo ; Lin, Zhihong ; Mattmann, Margrith E. ; Zou, Beiyan ; Terrenoire, Cecile ; Zhang, Hongkang ; Wu, Meng ; McManus, Owen B. ; Kass, Robert S. ; Lindsley, Craig W. ; Hopkins, Corey R. ; Li, Min. / Dynamic subunit stoichiometry confers a progressive continuum of pharmacological sensitivity by KCNQ potassium channels. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 21. pp. 8732-8737.
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AU - Zhang, Hongkang

AU - Wu, Meng

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