Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis

Irina V Agarkova, Brigitte Hertel, Xinzheng Zhang, Les Lane, Alexander Tchourbanov, David D Dunigan, Gerhard Thiel, Michael G. Rossmann, James L Van Etten

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Chloroviruses infect their hosts by specifically binding to and degrading the cell wall of their algal hosts at the site of attachment, using an intrinsic digesting enzyme(s). Chlorovirus PBCV-1 stored as a lysate survived longer than virus alone, suggesting virus attachment to cellular debris may be reversible. Ghost cells (algal cells extracted with methanol) were used as a model to study reversibility of PBCV-1 attachment because ghost cells are as susceptible to attachment and wall digestion as are live cells. Reversibility of attachment to ghost cells was examined by releasing attached virions with a cell wall degrading enzyme extract. The majority of the released virions retained infectivity even after re-incubating the released virions with ghost cells two times. Thus the chloroviruses appear to have a dynamic attachment strategy that may be beneficial in indigenous environments where cell wall debris can act as a refuge until appropriate host cells are available.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalVirology
Volume466-467
DOIs
StatePublished - Oct 1 2014

Fingerprint

Chlorella
Virion
Cell Wall
Virus Attachment
Enzymes
Methanol
Digestion
Viruses

Keywords

  • Cell wall degrading enzyme activity
  • Cell wall digestion
  • Chlorella
  • Chloroviruses
  • PBCV-1
  • Phycodnaviridae
  • Reversibility of virus attachment
  • Virus attachment
  • Virus infection
  • Virus survival

ASJC Scopus subject areas

  • Virology

Cite this

Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis. / Agarkova, Irina V; Hertel, Brigitte; Zhang, Xinzheng; Lane, Les; Tchourbanov, Alexander; Dunigan, David D; Thiel, Gerhard; Rossmann, Michael G.; Van Etten, James L.

In: Virology, Vol. 466-467, 01.10.2014, p. 95-102.

Research output: Contribution to journalArticle

Agarkova, IV, Hertel, B, Zhang, X, Lane, L, Tchourbanov, A, Dunigan, DD, Thiel, G, Rossmann, MG & Van Etten, JL 2014, 'Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis', Virology, vol. 466-467, pp. 95-102. https://doi.org/10.1016/j.virol.2014.07.002
Agarkova, Irina V ; Hertel, Brigitte ; Zhang, Xinzheng ; Lane, Les ; Tchourbanov, Alexander ; Dunigan, David D ; Thiel, Gerhard ; Rossmann, Michael G. ; Van Etten, James L. / Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis. In: Virology. 2014 ; Vol. 466-467. pp. 95-102.
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