Dynamic and differential regulation in the microRNA expression in the developing and mature cataractous rat lens

Eri Kubo, Nailia Hasanova, Hiroshi Sasaki, Dhirendra P. Singh

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Recent evidence supports a role for microRNAs (miRNAs) in regulating gene expression, and alterations in gene expression are known to affect cells involved in the development of ageing disorders. Using developing rat lens epithelial cells (LECs), we profiled the expression of miRNAs by a microarray-based approach. Few gene expression changes known to be involved in pathogenesis or cytoprotection were uniquely influenced by miRNA expression. Most miRNAs increased or decreased in abundance (let 7b, let 7c, miR29a, miR29c, miR126 and miR551b) in LECs/lenses during late embryonic and post-natal development and in cataract. Among them, miR29a, miR29c and miR126 were dramatically decreased in cataractous LECs from Shumiya Cataract Rats (SCRs). Specifically, the cytoskeleton remodelling genes tropomyosin (Tm) 1α and 2β, which have been implicated in the initiation of pathophysiology, were targets of miR29c and were over-stimulated as demonstrated by inhibitor experiments. In transfection experiments, increasing the level of miR29c caused a corresponding decrease in the expression of Tm1α and 2β, suggesting that miR29c may regulate the translation of Tm1α and 2β. 3′UTR luciferase activity of Tm1α, not 2β, was significantly decreased in miR29c-transfected mouse LECs. These findings demonstrate changes in miRNAs expression, and target molecules have potential as diagnostic indicators of ageing and as a foundation of miR-based therapeutics for age-related diseases.

Original languageEnglish (US)
Pages (from-to)1146-1159
Number of pages14
JournalJournal of cellular and molecular medicine
Volume17
Issue number9
DOIs
StatePublished - Sep 1 2013

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MicroRNAs
Lenses
Epithelial Cells
Gene Expression
Cataract
Tropomyosin
Cytoprotection
Cytoskeleton
Luciferases
Transfection
Genes
Therapeutics

Keywords

  • Ageing
  • Cataract
  • Lens development
  • MicroRNA
  • Tropomyosin

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

Cite this

Dynamic and differential regulation in the microRNA expression in the developing and mature cataractous rat lens. / Kubo, Eri; Hasanova, Nailia; Sasaki, Hiroshi; Singh, Dhirendra P.

In: Journal of cellular and molecular medicine, Vol. 17, No. 9, 01.09.2013, p. 1146-1159.

Research output: Contribution to journalArticle

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