Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation

Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier

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5 Citations (Scopus)

Abstract

Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA-NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA-NP complexes to substrates. The concentration of DNA-NP solutions influences the adsorbed DNA-NP surface mass, which increases by a factor of approximately 6 (detected by SE) and approximately 4.5-fold (detected by QCM-D), as the DNA concentration increases from 1.5 μg/mL to 15 μg/mL, with an increase in layer porosity. In addition, SE/QCM-D analysis indicates that DNA-NP adsorption rates, surface coverage densities, and volume fractions are dependent on the type of substrate: gold (Au) and silicon dioxide substrates, protein-coated and uncoated substrates, and surfaces modified with alkanethiol self assembled monolayers (SAMs). These studies also demonstrate that the influence of an adsorbed protein layer on resulting DNA-NP immobilization efficiency is substrate dependent. For example, Au surfaces coated with fetal bovine serum (FBS) resulted in two-fold greater mass of adsorbed DNA-NPs, compared to DNA-NP adsorption to FBS-coated SAM substrates. This investigation offers insights into dynamic DNA-NP surface adsorption processes, characteristics of the immobilized DNA-NP layer, and demonstrates substrate-dependent DNA-NP adsorption.

Original languageEnglish (US)
Pages (from-to)637-643
Number of pages7
JournalThin Solid Films
Volume571
Issue numberP3
DOIs
StatePublished - Nov 28 2014

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Spectroscopic ellipsometry
Quartz crystal microbalances
Biocompatible Materials
quartz crystals
immobilization
Biomaterials
microbalances
Dynamic analysis
ellipsometry
DNA
dissipation
deoxyribonucleic acid
Nanoparticles
nanoparticles
Substrates
crystals
Adsorption
adsorption
Immobilized Nucleic Acids
delivery

Keywords

  • Adsorption
  • DNA nanoparticles
  • Nonviral gene delivery
  • Quartz crystal microbalance with dissipation
  • Spectroscopic ellipsometry
  • Surface immobilization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

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title = "Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation",
abstract = "Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA-NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA-NP complexes to substrates. The concentration of DNA-NP solutions influences the adsorbed DNA-NP surface mass, which increases by a factor of approximately 6 (detected by SE) and approximately 4.5-fold (detected by QCM-D), as the DNA concentration increases from 1.5 μg/mL to 15 μg/mL, with an increase in layer porosity. In addition, SE/QCM-D analysis indicates that DNA-NP adsorption rates, surface coverage densities, and volume fractions are dependent on the type of substrate: gold (Au) and silicon dioxide substrates, protein-coated and uncoated substrates, and surfaces modified with alkanethiol self assembled monolayers (SAMs). These studies also demonstrate that the influence of an adsorbed protein layer on resulting DNA-NP immobilization efficiency is substrate dependent. For example, Au surfaces coated with fetal bovine serum (FBS) resulted in two-fold greater mass of adsorbed DNA-NPs, compared to DNA-NP adsorption to FBS-coated SAM substrates. This investigation offers insights into dynamic DNA-NP surface adsorption processes, characteristics of the immobilized DNA-NP layer, and demonstrates substrate-dependent DNA-NP adsorption.",
keywords = "Adsorption, DNA nanoparticles, Nonviral gene delivery, Quartz crystal microbalance with dissipation, Spectroscopic ellipsometry, Surface immobilization",
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year = "2014",
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language = "English (US)",
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TY - JOUR

T1 - Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation

AU - Kasputis, Tadas

AU - Pieper, Alex

AU - Schubert, Mathias

AU - Pannier, Angela K.

PY - 2014/11/28

Y1 - 2014/11/28

N2 - Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA-NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA-NP complexes to substrates. The concentration of DNA-NP solutions influences the adsorbed DNA-NP surface mass, which increases by a factor of approximately 6 (detected by SE) and approximately 4.5-fold (detected by QCM-D), as the DNA concentration increases from 1.5 μg/mL to 15 μg/mL, with an increase in layer porosity. In addition, SE/QCM-D analysis indicates that DNA-NP adsorption rates, surface coverage densities, and volume fractions are dependent on the type of substrate: gold (Au) and silicon dioxide substrates, protein-coated and uncoated substrates, and surfaces modified with alkanethiol self assembled monolayers (SAMs). These studies also demonstrate that the influence of an adsorbed protein layer on resulting DNA-NP immobilization efficiency is substrate dependent. For example, Au surfaces coated with fetal bovine serum (FBS) resulted in two-fold greater mass of adsorbed DNA-NPs, compared to DNA-NP adsorption to FBS-coated SAM substrates. This investigation offers insights into dynamic DNA-NP surface adsorption processes, characteristics of the immobilized DNA-NP layer, and demonstrates substrate-dependent DNA-NP adsorption.

AB - Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA-NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA-NP complexes to substrates. The concentration of DNA-NP solutions influences the adsorbed DNA-NP surface mass, which increases by a factor of approximately 6 (detected by SE) and approximately 4.5-fold (detected by QCM-D), as the DNA concentration increases from 1.5 μg/mL to 15 μg/mL, with an increase in layer porosity. In addition, SE/QCM-D analysis indicates that DNA-NP adsorption rates, surface coverage densities, and volume fractions are dependent on the type of substrate: gold (Au) and silicon dioxide substrates, protein-coated and uncoated substrates, and surfaces modified with alkanethiol self assembled monolayers (SAMs). These studies also demonstrate that the influence of an adsorbed protein layer on resulting DNA-NP immobilization efficiency is substrate dependent. For example, Au surfaces coated with fetal bovine serum (FBS) resulted in two-fold greater mass of adsorbed DNA-NPs, compared to DNA-NP adsorption to FBS-coated SAM substrates. This investigation offers insights into dynamic DNA-NP surface adsorption processes, characteristics of the immobilized DNA-NP layer, and demonstrates substrate-dependent DNA-NP adsorption.

KW - Adsorption

KW - DNA nanoparticles

KW - Nonviral gene delivery

KW - Quartz crystal microbalance with dissipation

KW - Spectroscopic ellipsometry

KW - Surface immobilization

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