Dur3 is the major urea transporter in Candida albicans and is co-regulated with the urea amidolyase Dur1,2

Dhammika H.M.L.P. Navarathna, Aditi Das, Joachim Morschhäuser, Kenneth W. Nickerson, David D. Roberts

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Hemiascomycetes, including the pathogen Candida albicans, acquire nitrogen from urea using the urea amidolyase Dur1,2, whereas all other higher fungi use primarily the nickel-containing urease. Urea metabolism via Dur1,2 is important for resistance to innate host immunity in C. albicans infections. To further characterize urea metabolism in C. albicans we examined the function of seven putative urea transporters. Gene disruption established that Dur3, encoded by orf 19.781, is the predominant transporter. [14C]Urea uptake was energy-dependent and decreased approximately sevenfold in a dur3D mutant. DUR1,2 and DUR3 expression was strongly induced by urea, whereas the other putative transporter genes were induced less than twofold. Immediate induction of DUR3 by urea was independent of its metabolism via Dur1,2, but further slow induction of DUR3 required the Dur1,2 pathway. We investigated the role of the GATA transcription factors Gat1 and Gln3 in DUR1,2 and DUR3 expression. Urea induction of DUR1,2 was reduced in a gat1Δ mutant, strongly reduced in a gln3Δ mutant, and abolished in a gat1Δ gln3Δ double mutant. In contrast, DUR3 induction by urea was preserved in both single mutants but reduced in the double mutant, suggesting that additional signaling mechanisms regulate DUR3 expression. These results establish Dur3 as the major urea transporter in C. albicans and provide additional insights into the control of urea utilization by this pathogen.

Original languageEnglish (US)
Pages (from-to)270-279
Number of pages10
JournalMicrobiology
Volume157
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Candida albicans
Urea
Contagious Ecthyma
GATA Transcription Factors
urea transporter
urea carboxylase (hydrolyzing)
Urease
Nickel
Innate Immunity
Genes
Fungi
Nitrogen
Infection

ASJC Scopus subject areas

  • Microbiology

Cite this

Dur3 is the major urea transporter in Candida albicans and is co-regulated with the urea amidolyase Dur1,2. / Navarathna, Dhammika H.M.L.P.; Das, Aditi; Morschhäuser, Joachim; Nickerson, Kenneth W.; Roberts, David D.

In: Microbiology, Vol. 157, No. 1, 01.01.2011, p. 270-279.

Research output: Contribution to journalArticle

Navarathna, Dhammika H.M.L.P. ; Das, Aditi ; Morschhäuser, Joachim ; Nickerson, Kenneth W. ; Roberts, David D. / Dur3 is the major urea transporter in Candida albicans and is co-regulated with the urea amidolyase Dur1,2. In: Microbiology. 2011 ; Vol. 157, No. 1. pp. 270-279.
@article{dc533dac464f4cc9b4cce4c588b8cf5e,
title = "Dur3 is the major urea transporter in Candida albicans and is co-regulated with the urea amidolyase Dur1,2",
abstract = "Hemiascomycetes, including the pathogen Candida albicans, acquire nitrogen from urea using the urea amidolyase Dur1,2, whereas all other higher fungi use primarily the nickel-containing urease. Urea metabolism via Dur1,2 is important for resistance to innate host immunity in C. albicans infections. To further characterize urea metabolism in C. albicans we examined the function of seven putative urea transporters. Gene disruption established that Dur3, encoded by orf 19.781, is the predominant transporter. [14C]Urea uptake was energy-dependent and decreased approximately sevenfold in a dur3D mutant. DUR1,2 and DUR3 expression was strongly induced by urea, whereas the other putative transporter genes were induced less than twofold. Immediate induction of DUR3 by urea was independent of its metabolism via Dur1,2, but further slow induction of DUR3 required the Dur1,2 pathway. We investigated the role of the GATA transcription factors Gat1 and Gln3 in DUR1,2 and DUR3 expression. Urea induction of DUR1,2 was reduced in a gat1Δ mutant, strongly reduced in a gln3Δ mutant, and abolished in a gat1Δ gln3Δ double mutant. In contrast, DUR3 induction by urea was preserved in both single mutants but reduced in the double mutant, suggesting that additional signaling mechanisms regulate DUR3 expression. These results establish Dur3 as the major urea transporter in C. albicans and provide additional insights into the control of urea utilization by this pathogen.",
author = "Navarathna, {Dhammika H.M.L.P.} and Aditi Das and Joachim Morschh{\"a}user and Nickerson, {Kenneth W.} and Roberts, {David D.}",
year = "2011",
month = "1",
day = "1",
doi = "10.1099/mic.0.045005-0",
language = "English (US)",
volume = "157",
pages = "270--279",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "1",

}

TY - JOUR

T1 - Dur3 is the major urea transporter in Candida albicans and is co-regulated with the urea amidolyase Dur1,2

AU - Navarathna, Dhammika H.M.L.P.

AU - Das, Aditi

AU - Morschhäuser, Joachim

AU - Nickerson, Kenneth W.

AU - Roberts, David D.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Hemiascomycetes, including the pathogen Candida albicans, acquire nitrogen from urea using the urea amidolyase Dur1,2, whereas all other higher fungi use primarily the nickel-containing urease. Urea metabolism via Dur1,2 is important for resistance to innate host immunity in C. albicans infections. To further characterize urea metabolism in C. albicans we examined the function of seven putative urea transporters. Gene disruption established that Dur3, encoded by orf 19.781, is the predominant transporter. [14C]Urea uptake was energy-dependent and decreased approximately sevenfold in a dur3D mutant. DUR1,2 and DUR3 expression was strongly induced by urea, whereas the other putative transporter genes were induced less than twofold. Immediate induction of DUR3 by urea was independent of its metabolism via Dur1,2, but further slow induction of DUR3 required the Dur1,2 pathway. We investigated the role of the GATA transcription factors Gat1 and Gln3 in DUR1,2 and DUR3 expression. Urea induction of DUR1,2 was reduced in a gat1Δ mutant, strongly reduced in a gln3Δ mutant, and abolished in a gat1Δ gln3Δ double mutant. In contrast, DUR3 induction by urea was preserved in both single mutants but reduced in the double mutant, suggesting that additional signaling mechanisms regulate DUR3 expression. These results establish Dur3 as the major urea transporter in C. albicans and provide additional insights into the control of urea utilization by this pathogen.

AB - Hemiascomycetes, including the pathogen Candida albicans, acquire nitrogen from urea using the urea amidolyase Dur1,2, whereas all other higher fungi use primarily the nickel-containing urease. Urea metabolism via Dur1,2 is important for resistance to innate host immunity in C. albicans infections. To further characterize urea metabolism in C. albicans we examined the function of seven putative urea transporters. Gene disruption established that Dur3, encoded by orf 19.781, is the predominant transporter. [14C]Urea uptake was energy-dependent and decreased approximately sevenfold in a dur3D mutant. DUR1,2 and DUR3 expression was strongly induced by urea, whereas the other putative transporter genes were induced less than twofold. Immediate induction of DUR3 by urea was independent of its metabolism via Dur1,2, but further slow induction of DUR3 required the Dur1,2 pathway. We investigated the role of the GATA transcription factors Gat1 and Gln3 in DUR1,2 and DUR3 expression. Urea induction of DUR1,2 was reduced in a gat1Δ mutant, strongly reduced in a gln3Δ mutant, and abolished in a gat1Δ gln3Δ double mutant. In contrast, DUR3 induction by urea was preserved in both single mutants but reduced in the double mutant, suggesting that additional signaling mechanisms regulate DUR3 expression. These results establish Dur3 as the major urea transporter in C. albicans and provide additional insights into the control of urea utilization by this pathogen.

UR - http://www.scopus.com/inward/record.url?scp=78650720285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650720285&partnerID=8YFLogxK

U2 - 10.1099/mic.0.045005-0

DO - 10.1099/mic.0.045005-0

M3 - Article

C2 - 20884691

AN - SCOPUS:78650720285

VL - 157

SP - 270

EP - 279

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 1

ER -