Dual-column determination of albumin and immunoglobulin G in serum by high-performance affinity chromatography

David S. Hage, Rodney R. Walters

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

High-performance affinity chromatography was used for the simultaneous determination of albumin and immunoglobulin G in serum. Two columns in series, the first containing immobilized anti-albumin antibodies and the other containing protein A for binding immunoglobulin G, were eluted separately at pH 3 by means of a column-switching system. This method gave results in good agreement with commercially available methods, while requiring only 2 μl of serum and 6.0 min per cycle. It was shown that albumin and immunoglobulin G were selectively retained, with little interference from other components, including immunoglobulins A and M.

Original languageEnglish (US)
Pages (from-to)37-49
Number of pages13
JournalJournal of Chromatography A
Volume386
Issue numberC
DOIs
StatePublished - 1987

Fingerprint

Affinity chromatography
Affinity Chromatography
Albumins
Immunoglobulin G
Serum
Immobilized Antibodies
Switching systems
Staphylococcal Protein A
Protein Binding
Immunoglobulin A
Immunoglobulin M
Anti-Idiotypic Antibodies
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Dual-column determination of albumin and immunoglobulin G in serum by high-performance affinity chromatography. / Hage, David S.; Walters, Rodney R.

In: Journal of Chromatography A, Vol. 386, No. C, 1987, p. 37-49.

Research output: Contribution to journalArticle

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