Drosophila homolog of the murine Int-1 protooncogene

E. Uzvolgyi, I. Kiss, A. Pitt, S. Arsenian, S. Ingvarsson, A. Udvardy, M. Hamada, G. Klein, Janos Sumegi

Research output: Contribution to journalArticle

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Abstract

We have isolated phage clones from Drosophila melanogaster genomic and cDNA libraries containing a sequence homologous to the murine Int-1 protooncogene. The Drosophila gene is represented by a single locus at position 28A1-2 on chromosome 2. The gene is expressed as a 2.9-kilobase-long polyadenylylated mRNA in embryo, larval, and pupal stages. It is hardly detectable in adult flies. The longest open reading frame of the cDNA clone corresponds to a protein 469 amino acids long. Alignment of the predicted amino acid sequences shows that the Drosophila protein is 86 amino acids longer than its murine counterpart. In spite of the difference in length, the two proteins are highly conserved with an overall sequence homology of 54%. Both Drosophila and murine Int-1 proteins beginm with a hydrophobic leader sequence and contain cysteine residues and sites for glycosylation (four in the murine protein and one in the Drosophila protein) in conserved positions, suggesting that they play important functional roles.

Original languageEnglish (US)
Pages (from-to)3034-3038
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number9
DOIs
StatePublished - Jan 1 1988

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Drosophila
Sequence Homology
Proteins
Clone Cells
Drosophila Proteins
Amino Acids
Chromosomes, Human, Pair 2
Genomic Library
Drosophila melanogaster
Gene Library
Glycosylation
Diptera
Bacteriophages
Open Reading Frames
Genes
Cysteine
Amino Acid Sequence
Embryonic Structures
Complementary DNA
Messenger RNA

ASJC Scopus subject areas

  • General

Cite this

Uzvolgyi, E., Kiss, I., Pitt, A., Arsenian, S., Ingvarsson, S., Udvardy, A., ... Sumegi, J. (1988). Drosophila homolog of the murine Int-1 protooncogene. Proceedings of the National Academy of Sciences of the United States of America, 85(9), 3034-3038. https://doi.org/10.1073/pnas.85.9.3034

Drosophila homolog of the murine Int-1 protooncogene. / Uzvolgyi, E.; Kiss, I.; Pitt, A.; Arsenian, S.; Ingvarsson, S.; Udvardy, A.; Hamada, M.; Klein, G.; Sumegi, Janos.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 9, 01.01.1988, p. 3034-3038.

Research output: Contribution to journalArticle

Uzvolgyi, E, Kiss, I, Pitt, A, Arsenian, S, Ingvarsson, S, Udvardy, A, Hamada, M, Klein, G & Sumegi, J 1988, 'Drosophila homolog of the murine Int-1 protooncogene', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 9, pp. 3034-3038. https://doi.org/10.1073/pnas.85.9.3034
Uzvolgyi, E. ; Kiss, I. ; Pitt, A. ; Arsenian, S. ; Ingvarsson, S. ; Udvardy, A. ; Hamada, M. ; Klein, G. ; Sumegi, Janos. / Drosophila homolog of the murine Int-1 protooncogene. In: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, No. 9. pp. 3034-3038.
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