Down-regulation of heme oxygenase-1 by hepatitis C virus infection in vivo and by the in vitro expression of hepatitis C core protein

Maher Y. Abdalla, Bradley E. Britigan, Feng Wen, Michael Icardi, Michael L. McCormick, Douglas R. LaBrecque, Michael Voigt, Kyle E. Brown, Warren N. Schmidt

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Antioxidant enzymes, including heme oxygenase (HO)-1, are an important line of defense against oxidant-mediated liver injury. Because hepatitis C virus (HCV) infection appears to increase the production of oxidants, we evaluated levels of antioxidant enzymes and HO-1 in liver-biopsy samples from HCV-infected patients by immunoblot and semiquantitative reverse-transcriptase polymerase chain reaction. In HCV-infected liver samples, levels of immunoreactive HO-1 and HO-1 mRNA were >4-fold lower than levels in control samples, but levels of superoxide dismutase and catalase were unaffected. Immunohistochemical results confirmed the decreased expression of HO-1 in hepatocytes from liver samples from HCV-infected patients but not in those from patients with other chronic liver diseases. The expression of HO-1 was also reduced in cell lines that stably express HCV core protein, which suggests that core gene products are capable of regulating the expression of HO-1.

Original languageEnglish (US)
Pages (from-to)1109-1118
Number of pages10
JournalJournal of Infectious Diseases
Volume190
Issue number6
DOIs
StatePublished - Sep 15 2004

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Heme Oxygenase-1
Virus Diseases
Hepatitis C
Hepacivirus
Down-Regulation
Proteins
Liver
Oxidants
Antioxidants
Enzymes
In Vitro Techniques
Reverse Transcriptase Polymerase Chain Reaction
Catalase
Superoxide Dismutase
Liver Diseases
Hepatocytes
Chronic Disease
Biopsy
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Down-regulation of heme oxygenase-1 by hepatitis C virus infection in vivo and by the in vitro expression of hepatitis C core protein. / Abdalla, Maher Y.; Britigan, Bradley E.; Wen, Feng; Icardi, Michael; McCormick, Michael L.; LaBrecque, Douglas R.; Voigt, Michael; Brown, Kyle E.; Schmidt, Warren N.

In: Journal of Infectious Diseases, Vol. 190, No. 6, 15.09.2004, p. 1109-1118.

Research output: Contribution to journalArticle

Abdalla, Maher Y. ; Britigan, Bradley E. ; Wen, Feng ; Icardi, Michael ; McCormick, Michael L. ; LaBrecque, Douglas R. ; Voigt, Michael ; Brown, Kyle E. ; Schmidt, Warren N. / Down-regulation of heme oxygenase-1 by hepatitis C virus infection in vivo and by the in vitro expression of hepatitis C core protein. In: Journal of Infectious Diseases. 2004 ; Vol. 190, No. 6. pp. 1109-1118.
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