Double-cuvette ISES: In situ estimation of enantioselectivity and relative rate for catalyst screening

Sangeeta Dey, Kannan R. Karukurichi, Weijun Shen, David B Berkowitz

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Described is a new method for the screening of an array of catalysts, in situ, to estimate enantioselectivity and relative rates. We term this approach "double-cuvette ISES (in situ enzymatic screening)". The Co(III)-salen mediated hydrolytic kinetic resolution (HKR) of (±)-propylene oxide is used as a model reaction to demonstrate proof of principle. In two parallel cuvettes, a lower CHCl3-based organic layer is loaded with the epoxide and the chiral salen catalyst. Aqueous reporting layers, containing distinct "reporting enzymes" and their nicotinamide cofactors, are layered above the organic layers. The 1,2-propanediol enantiomers formed by the chiral catalyst diffuse into the aqueous layer and are oxidized there by the reporting enzymes at rates dependent upon the diol concentration, the R:S ratio of the diol, and the enantioselectivity of the reporting enzymes. A focused chiral salen library was constructed from seven chiral 1,2-diamines, derived from amino acid, terpenoid, and carbohydrates skeletons, and seven salicylaldehyde derivatives. Double-cuvette ISES identified a couple of interesting combinatorial hits in this salen array, wherein either the sense or magnitude of enantioselection for a given chiral diamine depends significantly upon the choice of "salicylaldehyde" partner. A comparison of predicted ee's and relative rates using this new screening tool with those independently measured is provided.

Original languageEnglish (US)
Pages (from-to)8610-8611
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number24
DOIs
StatePublished - Jun 22 2005

Fingerprint

Enantioselectivity
Screening
Catalysts
Diamines
Enzymes
Propylene Glycol
Niacinamide
Enantiomers
Epoxy Compounds
Terpenes
Carbohydrates
Skeleton
Libraries
Propylene
Amino acids
Derivatives
Amino Acids
Kinetics
Oxides
disalicylaldehyde ethylenediamine

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Double-cuvette ISES : In situ estimation of enantioselectivity and relative rate for catalyst screening. / Dey, Sangeeta; Karukurichi, Kannan R.; Shen, Weijun; Berkowitz, David B.

In: Journal of the American Chemical Society, Vol. 127, No. 24, 22.06.2005, p. 8610-8611.

Research output: Contribution to journalArticle

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