Dose-dependent influences of ethanol on ischemic stroke

Role of inflammation

Guodong Xu, Chun Li, Anne L. Parsiola, Jiyu Li, Kimberly D. McCarter, Runhua Shi, William Mayhan, Hong Sun

Research output: Contribution to journalArticle

Abstract

Chronic ethanol consumption dose-dependently affects both incidence and prognosis of ischemic stroke. Our goal was to determine whether the influence of chronic ethanol consumption on ischemic stroke is related to an altered inflammatory profile in the brain. Male C57BL/6J mice were divided into six groups and gavage fed with 0.175, 0.35, 0.7, 1.4, 2.8 g/kg/day ethanol or volume-matched water once a day for 8 weeks. Adhesion molecules, microglial activation, neutrophil infiltration, pro- and anti-inflammatory cytokines/chemokines, blood-brain barrier (BBB) permeability, and matrix metallopeptidases (MMPs) in the cerebral cortex before and following a 90-min unilateral middle cerebral artery occlusion (MCAO)/24-h reperfusion were evaluated. Brain ischemia/reperfusion (I/R) injury was significantly reduced in 0.7 g/kg/day ethanol group (peak blood ethanol concentration: 9 mM) and worsened in 2.8 g/kg/day ethanol group (peak blood ethanol concentration: 37 mM). Baseline E-selectin was downregulated in all ethanol groups, whereas baseline intercellular adhesion molecule-1 (ICAM-1) was only downregulated in 0.35 and 0.7 g/kg/day ethanol groups. Interestingly, baseline vascular cell adhesion molecule-1 (VCAM-1) was upregulated in 0.35, 0.7, and 1.4 g/kg/day ethanol groups. Post-ischemic upregulation of ICAM-1 and E-selectin were suppressed in all ethanol groups. Post-ischemic neutrophil infiltration and microglial activation were significantly less in the low-moderate (0.1751.4 g/kg/day) ethanol groups but greater in the 2.8 g/kg/day ethanol group compared to the vehicle group. At basal conditions, ethanol increased one pro- and two anti-inflammatory cytokines/chemokines at the 0.7 g/kg/day dose, and 13 pro- and eight anti-inflammatory cytokines/chemokines at the 2.8 g/kg/day dose. After ischemia, 0.7 g/kg/day ethanol suppressed post-ischemic pro-inflammatory cytokines/chemokines and enhanced post-ischemic anti-inflammatory cytokines/chemokines. Moreover, 0.7 g/kg/day ethanol significantly reduced baseline MMP-9 activity and alleviated post-ischemic BBB breakdown. On the other hand, 2.8 g/kg/day ethanol worsened post-ischemic BBB breakdown. Our findings suggest that low-moderate ethanol consumption may prevent ischemic stroke and reduce brain I/R injury by suppressing inflammation, whereas heavy alcohol consumption may induce ischemic stroke and worsen brain I/R injury by aggravating inflammation.

Original languageEnglish (US)
Article number6
JournalFrontiers in Cellular Neuroscience
Volume13
DOIs
StatePublished - Jan 29 2019

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Ethanol
Stroke
Inflammation
Chemokines
Cytokines
Anti-Inflammatory Agents
Reperfusion Injury
Brain Ischemia
Blood-Brain Barrier
E-Selectin
Neutrophil Infiltration
Metalloproteases
Intercellular Adhesion Molecule-1
Blood Group Antigens
Down-Regulation
Vascular Cell Adhesion Molecule-1
Middle Cerebral Artery Infarction
Inbred C57BL Mouse
Alcohol Drinking
Cerebral Cortex

Keywords

  • Adhesion molecule
  • Cytokine/chemokine
  • Ethanol
  • Ischemic stroke
  • Matrix metalloproteinase
  • Microglia
  • Neutrophil infiltration

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Dose-dependent influences of ethanol on ischemic stroke : Role of inflammation. / Xu, Guodong; Li, Chun; Parsiola, Anne L.; Li, Jiyu; McCarter, Kimberly D.; Shi, Runhua; Mayhan, William; Sun, Hong.

In: Frontiers in Cellular Neuroscience, Vol. 13, 6, 29.01.2019.

Research output: Contribution to journalArticle

Xu, Guodong ; Li, Chun ; Parsiola, Anne L. ; Li, Jiyu ; McCarter, Kimberly D. ; Shi, Runhua ; Mayhan, William ; Sun, Hong. / Dose-dependent influences of ethanol on ischemic stroke : Role of inflammation. In: Frontiers in Cellular Neuroscience. 2019 ; Vol. 13.
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