Dominant-negative and knockdown approaches to studying PPAR activity

Sudhir Singh, Robert G Bennett

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.

Original languageEnglish (US)
Title of host publicationPeroxisome Proliferator-Activated Receptors (PPARs)
Subtitle of host publicationMethods and Protocols
EditorsMostafa Badr, Jihan Youssef
Pages87-98
Number of pages12
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume952
ISSN (Print)1064-3745

Fingerprint

Peroxisome Proliferator-Activated Receptors
Cell Communication
Small Interfering RNA
Molecular Biology
Gene Expression

Keywords

  • Adenovirus
  • Dominant-negative
  • Peroxisome proliferator-activated receptor
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Singh, S., & Bennett, R. G. (2013). Dominant-negative and knockdown approaches to studying PPAR activity. In M. Badr, & J. Youssef (Eds.), Peroxisome Proliferator-Activated Receptors (PPARs): Methods and Protocols (pp. 87-98). (Methods in Molecular Biology; Vol. 952). https://doi.org/10.1007/978-1-62703-155-4_6

Dominant-negative and knockdown approaches to studying PPAR activity. / Singh, Sudhir; Bennett, Robert G.

Peroxisome Proliferator-Activated Receptors (PPARs): Methods and Protocols. ed. / Mostafa Badr; Jihan Youssef. 2013. p. 87-98 (Methods in Molecular Biology; Vol. 952).

Research output: Chapter in Book/Report/Conference proceedingChapter

Singh, S & Bennett, RG 2013, Dominant-negative and knockdown approaches to studying PPAR activity. in M Badr & J Youssef (eds), Peroxisome Proliferator-Activated Receptors (PPARs): Methods and Protocols. Methods in Molecular Biology, vol. 952, pp. 87-98. https://doi.org/10.1007/978-1-62703-155-4_6
Singh S, Bennett RG. Dominant-negative and knockdown approaches to studying PPAR activity. In Badr M, Youssef J, editors, Peroxisome Proliferator-Activated Receptors (PPARs): Methods and Protocols. 2013. p. 87-98. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-155-4_6
Singh, Sudhir ; Bennett, Robert G. / Dominant-negative and knockdown approaches to studying PPAR activity. Peroxisome Proliferator-Activated Receptors (PPARs): Methods and Protocols. editor / Mostafa Badr ; Jihan Youssef. 2013. pp. 87-98 (Methods in Molecular Biology).
@inbook{724af72236cf4f5ba6caab429fd6ad07,
title = "Dominant-negative and knockdown approaches to studying PPAR activity",
abstract = "Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.",
keywords = "Adenovirus, Dominant-negative, Peroxisome proliferator-activated receptor, siRNA",
author = "Sudhir Singh and Bennett, {Robert G}",
year = "2013",
month = "1",
day = "1",
doi = "10.1007/978-1-62703-155-4_6",
language = "English (US)",
isbn = "9781627031547",
series = "Methods in Molecular Biology",
pages = "87--98",
editor = "Mostafa Badr and Jihan Youssef",
booktitle = "Peroxisome Proliferator-Activated Receptors (PPARs)",

}

TY - CHAP

T1 - Dominant-negative and knockdown approaches to studying PPAR activity

AU - Singh, Sudhir

AU - Bennett, Robert G

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.

AB - Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.

KW - Adenovirus

KW - Dominant-negative

KW - Peroxisome proliferator-activated receptor

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=84870560643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870560643&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-155-4_6

DO - 10.1007/978-1-62703-155-4_6

M3 - Chapter

SN - 9781627031547

T3 - Methods in Molecular Biology

SP - 87

EP - 98

BT - Peroxisome Proliferator-Activated Receptors (PPARs)

A2 - Badr, Mostafa

A2 - Youssef, Jihan

ER -