Domain interactions of the mannose 6-phosphate/insulin-like growth factor II receptor

Jodi L. Kreiling, James C. Byrd, Richard G MacDonald

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) forms oligomeric structures important for optimal function in binding and internalization of Man-6-P-bearing extracellular ligands as well as lysosomal biogenesis and growth regulation. However, neither the mechanism of inter-receptor interaction nor the dimerization domain has yet been identified. We hypothesized that areas near the ligand binding domains of the receptor would contribute preferentially to oligomerization. Two panels of minireceptors were constructed that involved truncations of either the N- or C-terminal regions of the M6P/IGF2R encompassing deletions of various ligand binding domains. α-FLAG or α-Myc-based immunoprecipitation assays showed that all of the minireceptors tested were able to associate with a full-length, Myc-tagged M6P/IGF2R (WT-M). In the α-FLAG but not α-Myc immunoprecipitation assays, the degree of association of a series of C-terminally truncated minireceptors with WT-M showed a positive trend with length of the minireceptor. In contrast, length did not seem to affect the association of the N-terminally truncated minireceptors with WT-M, except that the 12th extracytoplasmic repeat appeared exceptionally important in dimerization in the α-FLAG assays. The presence of mutations in the ligand-binding sites of the minireceptors had no effect on their ability to associate with WT-M. Thus, association within the heterodimers was not dependent on the presence of functional ligand binding domains. Heterodimers formed between WT-M and the minireceptors demonstrated high affinity IGF-II and Man-6-P-ligand binding, suggesting a functional association. We conclude that there is no finite M6P/IGF2R dimerization domain, but rather that interactions between dimer partners occur all along the extracytoplasmic region of the receptor.

Original languageEnglish (US)
Pages (from-to)21067-21077
Number of pages11
JournalJournal of Biological Chemistry
Volume280
Issue number22
DOIs
StatePublished - Jun 3 2005

Fingerprint

IGF Type 2 Receptor
Ligands
Dimerization
Association reactions
Assays
Immunoprecipitation
Bearings (structural)
Oligomerization
Insulin-Like Growth Factor II
Dimers
mannose-6-phosphate
Binding Sites
Mutation
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Domain interactions of the mannose 6-phosphate/insulin-like growth factor II receptor. / Kreiling, Jodi L.; Byrd, James C.; MacDonald, Richard G.

In: Journal of Biological Chemistry, Vol. 280, No. 22, 03.06.2005, p. 21067-21077.

Research output: Contribution to journalArticle

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