DNA uptake by type IV filaments

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Bacterial uptake of DNA through type IV filaments is an essential component of natural competence in numerous gram-positive and gram-negative species. Recent advances in the field have broadened our understanding of the structures used to take up extracellular DNA. Here, we review seminal experiments in the literature describing DNA binding by type IV pili, competence pili and the flp pili of Micrococcus luteus; collectively referred to here as type IV filaments. We compare the current state of the field on mechanisms of DNA uptake for these three appendage systems and describe the current mechanistic understanding of both DNA-binding and DNA-uptake by these versatile molecular machines.

Original languageEnglish (US)
Article number00001
JournalFrontiers in Molecular Biosciences
Volume6
Issue numberFEB
DOIs
StatePublished - Jan 1 2019

Fingerprint

DNA
Mental Competency
Micrococcus luteus
Bacterial DNA
Experiments

Keywords

  • Competence pili
  • DNA-binding
  • Flp pili
  • Horizontal gene transfer
  • Natural competence
  • Type IV pili

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

DNA uptake by type IV filaments. / Piepenbrink, Kurt H.

In: Frontiers in Molecular Biosciences, Vol. 6, No. FEB, 00001, 01.01.2019.

Research output: Contribution to journalReview article

@article{a7ca285a1a0345cb8d17db86bc71d4a0,
title = "DNA uptake by type IV filaments",
abstract = "Bacterial uptake of DNA through type IV filaments is an essential component of natural competence in numerous gram-positive and gram-negative species. Recent advances in the field have broadened our understanding of the structures used to take up extracellular DNA. Here, we review seminal experiments in the literature describing DNA binding by type IV pili, competence pili and the flp pili of Micrococcus luteus; collectively referred to here as type IV filaments. We compare the current state of the field on mechanisms of DNA uptake for these three appendage systems and describe the current mechanistic understanding of both DNA-binding and DNA-uptake by these versatile molecular machines.",
keywords = "Competence pili, DNA-binding, Flp pili, Horizontal gene transfer, Natural competence, Type IV pili",
author = "Piepenbrink, {Kurt H.}",
year = "2019",
month = "1",
day = "1",
doi = "10.3389/fmolb.2019.00001",
language = "English (US)",
volume = "6",
journal = "Frontiers in Molecular Biosciences",
issn = "2296-889X",
publisher = "Frontiers Media S. A.",
number = "FEB",

}

TY - JOUR

T1 - DNA uptake by type IV filaments

AU - Piepenbrink, Kurt H.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Bacterial uptake of DNA through type IV filaments is an essential component of natural competence in numerous gram-positive and gram-negative species. Recent advances in the field have broadened our understanding of the structures used to take up extracellular DNA. Here, we review seminal experiments in the literature describing DNA binding by type IV pili, competence pili and the flp pili of Micrococcus luteus; collectively referred to here as type IV filaments. We compare the current state of the field on mechanisms of DNA uptake for these three appendage systems and describe the current mechanistic understanding of both DNA-binding and DNA-uptake by these versatile molecular machines.

AB - Bacterial uptake of DNA through type IV filaments is an essential component of natural competence in numerous gram-positive and gram-negative species. Recent advances in the field have broadened our understanding of the structures used to take up extracellular DNA. Here, we review seminal experiments in the literature describing DNA binding by type IV pili, competence pili and the flp pili of Micrococcus luteus; collectively referred to here as type IV filaments. We compare the current state of the field on mechanisms of DNA uptake for these three appendage systems and describe the current mechanistic understanding of both DNA-binding and DNA-uptake by these versatile molecular machines.

KW - Competence pili

KW - DNA-binding

KW - Flp pili

KW - Horizontal gene transfer

KW - Natural competence

KW - Type IV pili

UR - http://www.scopus.com/inward/record.url?scp=85064458339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064458339&partnerID=8YFLogxK

U2 - 10.3389/fmolb.2019.00001

DO - 10.3389/fmolb.2019.00001

M3 - Review article

C2 - 30805346

AN - SCOPUS:85064458339

VL - 6

JO - Frontiers in Molecular Biosciences

JF - Frontiers in Molecular Biosciences

SN - 2296-889X

IS - FEB

M1 - 00001

ER -