DNA synthesis of mononuclear cells exposed to lansoprazole, omeprazole, and ranitidine

T. E. Peddicord, D. S. Collier, K. M. Olsen

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Abstract

DNA synthesis in mononuclear cells (MC) exposed to lansoprazole (LA), omeprazole (OM), and ranitidine (RA) was measured using 3H-thymidine (Tdr) uptake. Venous blood was collected from normal subjects and MC were isolated using centrifugation techniques over a Ficoll-Hypaque density gradient. MC were added to 12-well culture plates in 4 groups; control (C), LA (25 μg/mL), OM (0.35 μg/mL), and RA (50 μg/mL). MC were exposed to drug for 96 hrs, with addition of phytohemagglutinin (1 μg/mL) for the last 48 hrs, and Tdr (1 μCi) the final 6 hrs. Cells were filtered onto glass fiber filter paper, rinsed, and counted on a SC counter. Data were analyzed by ANOVA and Tukey's HSD Test. Mean disintegrations per minute/MC × 10-4 ± SEM are reported: C 61.85 ± 9.1, RA 24.42 ± 2.7, LA 11.59 ± 1.3, OM 13.97 ± 3.6. C showed greater Tdr uptake (p <0.001) than OM, LA, and RA. No differences were observed between OM, LA, and RA, but trends of lower Tdr uptake were observed in OM and LA. This is one of the first studies to examine the effect of proton pump inhibitors on human mononuclear cell DNA synthesis. Lansoprazole, omeprazole, and ranitidine decreased DNA synthesis of mononuclear cells when compared to control.

Original languageEnglish (US)
Number of pages1
JournalClinical Pharmacology and Therapeutics
Volume61
Issue number2
StatePublished - Dec 1 1997

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ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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