DNA Sequences Tightly Bound to Proteins in Mouse Chromatin: Identification of Murine MER Sequences

Zoya Avramova, Oleg Georgiev, Roumen Tsanev

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The finding of stably (tightly) associated DNA–protein complexes in eukaryotic chromatin has provoked many hypotheses and speculations concerning their possible role. While the answer of this question is not envisaged yet, it is clear that elucidation of the nature of the individual components involved in such complexes is a necessary step in this direction. Here, the nature of several mouse DNA sequences in the vicinity of a putative stably attached protein is studied. Eight independently isolated clones containing such sequences were compared to known sequences in GenBank. Two clones were found to belong to different subfamilies of repetitive sequences, organized into a larger family — the L1md family. One clone harbors a sequence that is a member of the Alu-type family. Four of the cloned sequences are preset in low copy numbers, but the computer search found similar sequences in various genomic regions of different rodents. These facts, together with the finding that regions homologous to the above clones often flank other repetitive elements in the genome, suggest that the cloned sequences belong to new, not yet described families of repeats in the murine genome. It is possible that they correspond to the medium reiteration frequency sequences, MER-sequences, discovered recently in the human genome (Jurka, 1990; Kaplan and Duncan, 1990). Particularly intriguing is the homology found at the integration sites of polyoma virus in two transformed cell lines with two of these clones.

Original languageEnglish (US)
Pages (from-to)539-548
Number of pages10
JournalDNA and Cell Biology
Volume13
Issue number5
DOIs
StatePublished - May 1994

Fingerprint

Chromatin
Clone Cells
Proteins
Alu Elements
Genome
Polyomavirus
Transformed Cell Line
Nucleic Acid Repetitive Sequences
Nucleic Acid Databases
Human Genome
Rodentia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

DNA Sequences Tightly Bound to Proteins in Mouse Chromatin : Identification of Murine MER Sequences. / Avramova, Zoya; Georgiev, Oleg; Tsanev, Roumen.

In: DNA and Cell Biology, Vol. 13, No. 5, 05.1994, p. 539-548.

Research output: Contribution to journalArticle

@article{290ab02d46894b848588e156cbed832e,
title = "DNA Sequences Tightly Bound to Proteins in Mouse Chromatin: Identification of Murine MER Sequences",
abstract = "The finding of stably (tightly) associated DNA–protein complexes in eukaryotic chromatin has provoked many hypotheses and speculations concerning their possible role. While the answer of this question is not envisaged yet, it is clear that elucidation of the nature of the individual components involved in such complexes is a necessary step in this direction. Here, the nature of several mouse DNA sequences in the vicinity of a putative stably attached protein is studied. Eight independently isolated clones containing such sequences were compared to known sequences in GenBank. Two clones were found to belong to different subfamilies of repetitive sequences, organized into a larger family — the L1md family. One clone harbors a sequence that is a member of the Alu-type family. Four of the cloned sequences are preset in low copy numbers, but the computer search found similar sequences in various genomic regions of different rodents. These facts, together with the finding that regions homologous to the above clones often flank other repetitive elements in the genome, suggest that the cloned sequences belong to new, not yet described families of repeats in the murine genome. It is possible that they correspond to the medium reiteration frequency sequences, MER-sequences, discovered recently in the human genome (Jurka, 1990; Kaplan and Duncan, 1990). Particularly intriguing is the homology found at the integration sites of polyoma virus in two transformed cell lines with two of these clones.",
author = "Zoya Avramova and Oleg Georgiev and Roumen Tsanev",
year = "1994",
month = "5",
doi = "10.1089/dna.1994.13.539",
language = "English (US)",
volume = "13",
pages = "539--548",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - DNA Sequences Tightly Bound to Proteins in Mouse Chromatin

T2 - Identification of Murine MER Sequences

AU - Avramova, Zoya

AU - Georgiev, Oleg

AU - Tsanev, Roumen

PY - 1994/5

Y1 - 1994/5

N2 - The finding of stably (tightly) associated DNA–protein complexes in eukaryotic chromatin has provoked many hypotheses and speculations concerning their possible role. While the answer of this question is not envisaged yet, it is clear that elucidation of the nature of the individual components involved in such complexes is a necessary step in this direction. Here, the nature of several mouse DNA sequences in the vicinity of a putative stably attached protein is studied. Eight independently isolated clones containing such sequences were compared to known sequences in GenBank. Two clones were found to belong to different subfamilies of repetitive sequences, organized into a larger family — the L1md family. One clone harbors a sequence that is a member of the Alu-type family. Four of the cloned sequences are preset in low copy numbers, but the computer search found similar sequences in various genomic regions of different rodents. These facts, together with the finding that regions homologous to the above clones often flank other repetitive elements in the genome, suggest that the cloned sequences belong to new, not yet described families of repeats in the murine genome. It is possible that they correspond to the medium reiteration frequency sequences, MER-sequences, discovered recently in the human genome (Jurka, 1990; Kaplan and Duncan, 1990). Particularly intriguing is the homology found at the integration sites of polyoma virus in two transformed cell lines with two of these clones.

AB - The finding of stably (tightly) associated DNA–protein complexes in eukaryotic chromatin has provoked many hypotheses and speculations concerning their possible role. While the answer of this question is not envisaged yet, it is clear that elucidation of the nature of the individual components involved in such complexes is a necessary step in this direction. Here, the nature of several mouse DNA sequences in the vicinity of a putative stably attached protein is studied. Eight independently isolated clones containing such sequences were compared to known sequences in GenBank. Two clones were found to belong to different subfamilies of repetitive sequences, organized into a larger family — the L1md family. One clone harbors a sequence that is a member of the Alu-type family. Four of the cloned sequences are preset in low copy numbers, but the computer search found similar sequences in various genomic regions of different rodents. These facts, together with the finding that regions homologous to the above clones often flank other repetitive elements in the genome, suggest that the cloned sequences belong to new, not yet described families of repeats in the murine genome. It is possible that they correspond to the medium reiteration frequency sequences, MER-sequences, discovered recently in the human genome (Jurka, 1990; Kaplan and Duncan, 1990). Particularly intriguing is the homology found at the integration sites of polyoma virus in two transformed cell lines with two of these clones.

UR - http://www.scopus.com/inward/record.url?scp=0028021229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028021229&partnerID=8YFLogxK

U2 - 10.1089/dna.1994.13.539

DO - 10.1089/dna.1994.13.539

M3 - Article

C2 - 8024697

AN - SCOPUS:0028021229

VL - 13

SP - 539

EP - 548

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 5

ER -