DNA sequence analysis of regions surrounding blaCMY-2 from multiple Salmonella plasmid backbones

W. P. Giles, Andrew K Benson, M. E. Olson, Robert W Hutkins, J. M. Whichard, P. L. Winokur, Paul D Fey

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Abstract

The emergence in the United States of resistance to expanded-spectrum cephalosporin (e.g., ceftriaxone) within the salmonellae has been associated primarily with three large (>100-kb) plasmids (designated types A, B, and C) and one 10.1-kb plasmid (type D) that carry the blaCMY-2 gene. In the present study, the distribution of these four known blaCMY-2- carrying plasmids among 35 ceftriaxone-resistant Salmonella isolates obtained from 1998 to 2001 was examined. Twenty-three of these isolates were Salmonella enterica serotype Newport, 10 were Salmonella enterica serotype Typhimurium, 1 was Salmonella enterica serotype Agona, and 1 was Salmonella enterica serotype Reading. All 23 serotype Newport isolates carried a type C plasmid, and 5, 4, and 1 serovar Typhimurium isolate carried type B, A, and C plasmids, respectively. Both the serotype Agona and serotype Reading isolates carried type A plasmids. None of the isolates carried a type D plasmid. Hybridization data suggested that plasmid types A and C were highly related replicons. DNA sequencing revealed that the region surrounding blaCMY-2 was highly conserved in all three plasmid types analyzed (types B, C, and D) and was related to a region surrounding blaCMY-2 from the Klebsiella oxytoca plasmid pTKH11. These findings are consistent with a model in which bla CMY-2 has been disseminated primarily through plasmid transfer, and not by mobilization of the gene itself, to multiple Salmonella chromosomal backbones.

Original languageEnglish (US)
Pages (from-to)2845-2852
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume48
Issue number8
DOIs
StatePublished - Aug 1 2004

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DNA Sequence Analysis
Salmonella
Plasmids
Salmonella enterica
Ceftriaxone
Reading
Klebsiella oxytoca
Replicon
Serogroup
Cephalosporins
Genes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

DNA sequence analysis of regions surrounding blaCMY-2 from multiple Salmonella plasmid backbones. / Giles, W. P.; Benson, Andrew K; Olson, M. E.; Hutkins, Robert W; Whichard, J. M.; Winokur, P. L.; Fey, Paul D.

In: Antimicrobial Agents and Chemotherapy, Vol. 48, No. 8, 01.08.2004, p. 2845-2852.

Research output: Contribution to journalArticle

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