DNA polymorphism and molecular subtyping of the capsular gene cluster of group B Streptococcus

Shannon D. Manning, David W. Lacher, Herbert Dele Davies, Betsy Foxman, Thomas S. Whittam

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.

Original languageEnglish (US)
Pages (from-to)6113-6116
Number of pages4
JournalJournal of clinical microbiology
Volume43
Issue number12
DOIs
StatePublished - Dec 1 2005

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Streptococcus agalactiae
Multigene Family
Polysaccharides
Serotyping
DNA
Genes
Digestion
Polymerase Chain Reaction
Enzymes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

DNA polymorphism and molecular subtyping of the capsular gene cluster of group B Streptococcus. / Manning, Shannon D.; Lacher, David W.; Davies, Herbert Dele; Foxman, Betsy; Whittam, Thomas S.

In: Journal of clinical microbiology, Vol. 43, No. 12, 01.12.2005, p. 6113-6116.

Research output: Contribution to journalArticle

Manning, Shannon D. ; Lacher, David W. ; Davies, Herbert Dele ; Foxman, Betsy ; Whittam, Thomas S. / DNA polymorphism and molecular subtyping of the capsular gene cluster of group B Streptococcus. In: Journal of clinical microbiology. 2005 ; Vol. 43, No. 12. pp. 6113-6116.
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