Abstract
DNA interstrand cross-links (ICLs) are mainly repaired by the combined action of nucleotide excision repair and homologous recombination in E. coli. Genetic data also suggest the existence of a nucleotide excision repair-dependent, homologous recombination-independent ICL repair pathway. The involvement of translesion synthesis in this pathway has been postulated; however, the molecular mechanism of this pathway is not understood. To examine the role of translesion synthesis in ICL repair, we generated a defined substrate with a single psoralen ICL that mimics a postincision structure generated by nucleotide excision repair. We demonstrated that the Klenow fragment (DNA polymerase I) performs translesion synthesis on this model substrate. This in vitro translesion synthesis assay will help in understanding the basic mechanism of a postincision translesion synthesis process in ICL repair.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5460-5464 |
| Number of pages | 5 |
| Journal | Biochemistry |
| Volume | 47 |
| Issue number | 19 |
| DOIs | |
| State | Published - May 13 2008 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
DNA polymerase I-mediated translesion synthesis in RecA-independent DNA interstrand cross-link Repair in E. coli. / Zietlow, Laura; Bessho, Tadayoshi.
In: Biochemistry, Vol. 47, No. 19, 13.05.2008, p. 5460-5464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA polymerase I-mediated translesion synthesis in RecA-independent DNA interstrand cross-link Repair in E. coli
AU - Zietlow, Laura
AU - Bessho, Tadayoshi
PY - 2008/5/13
Y1 - 2008/5/13
N2 - DNA interstrand cross-links (ICLs) are mainly repaired by the combined action of nucleotide excision repair and homologous recombination in E. coli. Genetic data also suggest the existence of a nucleotide excision repair-dependent, homologous recombination-independent ICL repair pathway. The involvement of translesion synthesis in this pathway has been postulated; however, the molecular mechanism of this pathway is not understood. To examine the role of translesion synthesis in ICL repair, we generated a defined substrate with a single psoralen ICL that mimics a postincision structure generated by nucleotide excision repair. We demonstrated that the Klenow fragment (DNA polymerase I) performs translesion synthesis on this model substrate. This in vitro translesion synthesis assay will help in understanding the basic mechanism of a postincision translesion synthesis process in ICL repair.
AB - DNA interstrand cross-links (ICLs) are mainly repaired by the combined action of nucleotide excision repair and homologous recombination in E. coli. Genetic data also suggest the existence of a nucleotide excision repair-dependent, homologous recombination-independent ICL repair pathway. The involvement of translesion synthesis in this pathway has been postulated; however, the molecular mechanism of this pathway is not understood. To examine the role of translesion synthesis in ICL repair, we generated a defined substrate with a single psoralen ICL that mimics a postincision structure generated by nucleotide excision repair. We demonstrated that the Klenow fragment (DNA polymerase I) performs translesion synthesis on this model substrate. This in vitro translesion synthesis assay will help in understanding the basic mechanism of a postincision translesion synthesis process in ICL repair.
UR - http://www.scopus.com/inward/record.url?scp=43249112839&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43249112839&partnerID=8YFLogxK
U2 - 10.1021/bi702343y
DO - 10.1021/bi702343y
M3 - Article
C2 - 18416557
AN - SCOPUS:43249112839
VL - 47
SP - 5460
EP - 5464
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 19
ER -