DNA polymerase alpha from the nuclear matrix of cells infected with simian virus 40

Clint Jones, Robert T. Su

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The nuclear matrix prepared from normal, simian virus 40 (SV40)-infected, and SV40-transformed cells contained DNA polymerase activities. Approximately 12% of the total DNA polymerase activities in isolated nuclei remained with the nuclear matrix, α-polymerase was the major matrix DNA polymerase activity as judged by sensitivity to various inhibitors: aphidicolin, dideoxy-TTP, and N-ethylmaleimide. Approximately 2-4 fold higher DNA polymerase activity was detected in matrices obtained from lytically infected and virus-transformed cells than that found in normal cells. In lytically infected cells, 30-50% of the matrix-bound DNA polymerase activity solubilized by sonication co-sedimented with majority of the matrix T-antigen, and was co-precipitated with anti-T sera. The results suggest that α-polymerase and viral T-antigen may form a functional complex in the matrix.

Original languageEnglish (US)
Pages (from-to)5517-5532
Number of pages16
JournalNucleic acids research
Volume10
Issue number18
DOIs
StatePublished - Sep 25 1982

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Nuclear Matrix
DNA Polymerase I
Simian virus 40
DNA-Directed DNA Polymerase
Viruses
Virus
DNA
Cell
Viral Tumor Antigens
Antigens
Aphidicolin
Sonication
Ethylmaleimide
Inhibitor
Nucleus
Fold
Serum

ASJC Scopus subject areas

  • Genetics

Cite this

DNA polymerase alpha from the nuclear matrix of cells infected with simian virus 40. / Jones, Clint; Su, Robert T.

In: Nucleic acids research, Vol. 10, No. 18, 25.09.1982, p. 5517-5532.

Research output: Contribution to journalArticle

Jones, Clint ; Su, Robert T. / DNA polymerase alpha from the nuclear matrix of cells infected with simian virus 40. In: Nucleic acids research. 1982 ; Vol. 10, No. 18. pp. 5517-5532.
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