DNA polymerase alpha from the nuclear matrix of cells infected with simian virus 40

Clint Jones, Robert T. Su

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Abstract

The nuclear matrix prepared from normal, simian virus 40 (SV40)-infected, and SV40-transformed cells contained DNA polymerase activities. Approximately 12% of the total DNA polymerase activities in isolated nuclei remained with the nuclear matrix, α-polymerase was the major matrix DNA polymerase activity as judged by sensitivity to various inhibitors: aphidicolin, dideoxy-TTP, and N-ethylmaleimide. Approximately 2-4 fold higher DNA polymerase activity was detected in matrices obtained from lytically infected and virus-transformed cells than that found in normal cells. In lytically infected cells, 30-50% of the matrix-bound DNA polymerase activity solubilized by sonication co-sedimented with majority of the matrix T-antigen, and was co-precipitated with anti-T sera. The results suggest that α-polymerase and viral T-antigen may form a functional complex in the matrix.

Original languageEnglish (US)
Pages (from-to)5517-5532
Number of pages16
JournalNucleic acids research
Volume10
Issue number18
DOIs
Publication statusPublished - Sep 25 1982

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ASJC Scopus subject areas

  • Genetics

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