DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga

James L. van Etten, Anne M. Schuster, Lois Girton, Dwight E. Burbank, David Swinton, Stanley Hattman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six of the viral DNAs also contain N6-methyldeoxyadenosine. At least some of the methylated bases in the host reside in different base sequences than the nethylated bases in the viruses as shown by differential susceptibility to restriction endonuclease enzymes. This suggests that the viruses encode for DNA methyltransferases with sequence specificities different from that of the host enzyme.

Original languageEnglish (US)
Pages (from-to)3471-3478
Number of pages8
JournalNucleic acids research
Volume13
Issue number10
DOIs
StatePublished - May 24 1985

Fingerprint

Green Algae
Chlorella
Chlorophyta
5-methyldeoxycytidine
DNA Viruses
DNA Methylation
Algae
Viruses
Virus
DNA
Host Specificity
DNA Restriction Enzymes
Viral DNA
Methyltransferases
Enzymes
Susceptibility
Specificity
Genomics
Restriction

ASJC Scopus subject areas

  • Genetics

Cite this

van Etten, J. L., Schuster, A. M., Girton, L., Burbank, D. E., Swinton, D., & Hattman, S. (1985). DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucleic acids research, 13(10), 3471-3478. https://doi.org/10.1093/nar/13.10.3471

DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. / van Etten, James L.; Schuster, Anne M.; Girton, Lois; Burbank, Dwight E.; Swinton, David; Hattman, Stanley.

In: Nucleic acids research, Vol. 13, No. 10, 24.05.1985, p. 3471-3478.

Research output: Contribution to journalArticle

van Etten, JL, Schuster, AM, Girton, L, Burbank, DE, Swinton, D & Hattman, S 1985, 'DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga', Nucleic acids research, vol. 13, no. 10, pp. 3471-3478. https://doi.org/10.1093/nar/13.10.3471
van Etten, James L. ; Schuster, Anne M. ; Girton, Lois ; Burbank, Dwight E. ; Swinton, David ; Hattman, Stanley. / DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. In: Nucleic acids research. 1985 ; Vol. 13, No. 10. pp. 3471-3478.
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