DNA barcoding evidence for the North American presence of alfalfa cyst nematode, Heterodera medicaginis

Thomas Powers, Andrea Skantar, Tim Harris, Rebecca Higgins, Peter Mullin, Saad Hafez, Zafar Handoo, Tim Todd, Kirsten Powers

Research output: Contribution to journalArticle

Abstract

Specimens of Heterodera have been collected from alfalfa fields in Kearny County, Kansas and Carbon County, Montana. DNA barcoding with the COI mitochondrial gene indicate that the species is not Heterodera glycines, soybean cyst nematode, H. schachtii, sugar beet cyst nematode, or H. trifolii, clover cyst nematode. Maximum likelihood phylogenetic trees show that the alfalfa specimens form a sister clade most closely related to H. glycines, with a 4.7% mean pairwise sequence divergence across the 862 nucleotides of the COI marker. Morphological analyses of juveniles and cysts conform to the measurements of H. medicaginis, the alfalfa cyst nematode originally described from the USSR in 1971. Initial host testing demonstrated that the nematode reproduced on alfalfa, but not on soybeans, tomato, or corn. Collectively, the evidence suggests that this finding represents the first record of H. medicaginis in North America. Definitive confirmation of this diagnosis would require COI sequence of eastern European isolates of this species.

Original languageEnglish (US)
JournalJournal of Nematology
Volume51
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Heterodera
cyst nematodes
DNA barcoding
Heterodera glycines
alfalfa
Heterodera trifolii
Heterodera schachtii
USSR
sugar beet
nucleotides
Nematoda
tomatoes
soybeans
corn
carbon
phylogeny
genes
testing

Keywords

  • Alfalfa cyst nematode
  • COI DNA barcode
  • Detection
  • Diagnosis
  • Heterodera medicaginis
  • Taxonomy

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

DNA barcoding evidence for the North American presence of alfalfa cyst nematode, Heterodera medicaginis. / Powers, Thomas; Skantar, Andrea; Harris, Tim; Higgins, Rebecca; Mullin, Peter; Hafez, Saad; Handoo, Zafar; Todd, Tim; Powers, Kirsten.

In: Journal of Nematology, Vol. 51, No. 1, 01.01.2019.

Research output: Contribution to journalArticle

Powers, T, Skantar, A, Harris, T, Higgins, R, Mullin, P, Hafez, S, Handoo, Z, Todd, T & Powers, K 2019, 'DNA barcoding evidence for the North American presence of alfalfa cyst nematode, Heterodera medicaginis', Journal of Nematology, vol. 51, no. 1. https://doi.org/10.21307/jofnem-2019-016
Powers, Thomas ; Skantar, Andrea ; Harris, Tim ; Higgins, Rebecca ; Mullin, Peter ; Hafez, Saad ; Handoo, Zafar ; Todd, Tim ; Powers, Kirsten. / DNA barcoding evidence for the North American presence of alfalfa cyst nematode, Heterodera medicaginis. In: Journal of Nematology. 2019 ; Vol. 51, No. 1.
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