DNA barcoding distinguishes pest species of the black fly genus Cnephia (Diptera

Simuliidae)

I. M. Conflitti, K. P. Pruess, A. Cywinska, Thomas O Powers, D. C. Currie

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Accurate species identification is essential for cost-effective pest control strategies. We tested the utility of COI barcodes for identifying members of the black fly genus Cnephia Enderlein (Diptera: Simuliidae). Our efforts focus on four Nearctic Cnephia species - Cnephia dacotensis (Dyar & Shannon), Cnephia eremities Shewell, Cnephia ornithophilia (Davies, Peterson & Wood), and Cnephia pecuarum (Riley) - the latter two being current or potential targets of biological control programs. We also analyzed one Palearctic species, Cnephia pallipes (Fries). Although Cnephia adults can be identified anatomically to species, control programs target the larval stage, which is difficult or impossible to distinguish morphologically. By using neighborjoining, maximum parsimony, and Bayesian methods, we found that COI barcodes successfully identified three Nearctic Cnephia species, but not C. pecuarum. The Palearctic C. pallipes was also successfully identified. Despite nonmonophyly of C. pecuarum, we show that data from COI barcoding, in combination with geographical and ecological information, can be used to distinguish all four Nearctic species. Finally, we discussed 1) possible reasons for paraphyly in C. pecuarum, 2) topological concordance to previously reported chromosomal dendrograms, and 3) evolution of diverse feeding strategies within the genus Cnephia.

Original languageEnglish (US)
Pages (from-to)1250-1260
Number of pages11
JournalJournal of medical entomology
Volume50
Issue number6
DOIs
StatePublished - Nov 1 2013

Fingerprint

Cnephia
Simuliidae
DNA barcoding
Diptera
Cnephia pecuarum
pests
Pest Control
Bayes Theorem
DNA
barcoding
Costs and Cost Analysis
feeding methods
paraphyly
Bayesian theory
pest control
biological control

Keywords

  • Biological control
  • COI gene
  • Mitochondrial DNA
  • Monophyly
  • Paraphyly

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

DNA barcoding distinguishes pest species of the black fly genus Cnephia (Diptera : Simuliidae). / Conflitti, I. M.; Pruess, K. P.; Cywinska, A.; Powers, Thomas O; Currie, D. C.

In: Journal of medical entomology, Vol. 50, No. 6, 01.11.2013, p. 1250-1260.

Research output: Contribution to journalArticle

Conflitti, I. M. ; Pruess, K. P. ; Cywinska, A. ; Powers, Thomas O ; Currie, D. C. / DNA barcoding distinguishes pest species of the black fly genus Cnephia (Diptera : Simuliidae). In: Journal of medical entomology. 2013 ; Vol. 50, No. 6. pp. 1250-1260.
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