DL-3-n-Butylphthalide attenuation of methamphetamine-induced neurotoxicity in SH-SY5Y neuroblastoma cells

Jun Zhao, Jianuo Liu, Enquan Xu, Yunlin Liu, Anmu Xie, Huangui Xiong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims Methamphetamine (Meth) abuse causes neural injury in the brain. There are no efficacious therapies available to treat Meth-induce neural injury. The present study intended to test the therapeutic potential of DL-3-n-butylphthalide (NBP), a chemical compound extracted originally from the seeds of Chinese Celery, in the amelioration and prevention of Meth-induced neural injury. Main methods Experiments were carried out on SH-SY5Y cells. Neuronal injury and apoptotic cell death were detected by MTT assay and analysis of nuclear morphology. Intracellular reactive oxygen species (ROS) was evaluated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay and protein expression levels of the apoptosis-related cleaved caspase-3, bcl2 and Bax were determined by western blotting. Key findings Treatment of SH-SY5Y cells with Meth induced cell injury and apoptosis. NBP attenuated Meth-associated cell injury and apoptosis via blockage of Meth-mediated upregulation of intracellular ROS production and inhibition of Meth-induced decrease of cleaved caspase-3/caspase-3 and Bcl-2/Bax ratios. Significance The results presented in this study indicate that NBP may have therapeutic benefits in the treatment of Meth-induced neuronal injury in the central nervous system.

Original languageEnglish (US)
Pages (from-to)16-20
Number of pages5
JournalLife Sciences
Volume165
DOIs
StatePublished - Nov 15 2016

Fingerprint

Methamphetamine
Neuroblastoma
Wounds and Injuries
Caspase 3
Apoptosis
Assays
Reactive Oxygen Species
Apium graveolens
Chemical compounds
3-n-butylphthalide
Neurology
Cell death
Brain Injuries
Seed
Brain
Seeds
Cell Death
Up-Regulation
Therapeutics
Central Nervous System

Keywords

  • DL-3-n-Butylphthalide
  • Methamphetamine
  • Neuroprotection
  • Neurotoxicity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

DL-3-n-Butylphthalide attenuation of methamphetamine-induced neurotoxicity in SH-SY5Y neuroblastoma cells. / Zhao, Jun; Liu, Jianuo; Xu, Enquan; Liu, Yunlin; Xie, Anmu; Xiong, Huangui.

In: Life Sciences, Vol. 165, 15.11.2016, p. 16-20.

Research output: Contribution to journalArticle

Zhao, Jun ; Liu, Jianuo ; Xu, Enquan ; Liu, Yunlin ; Xie, Anmu ; Xiong, Huangui. / DL-3-n-Butylphthalide attenuation of methamphetamine-induced neurotoxicity in SH-SY5Y neuroblastoma cells. In: Life Sciences. 2016 ; Vol. 165. pp. 16-20.
@article{f6601bd8c02b4f01b0856368022aee1a,
title = "DL-3-n-Butylphthalide attenuation of methamphetamine-induced neurotoxicity in SH-SY5Y neuroblastoma cells",
abstract = "Aims Methamphetamine (Meth) abuse causes neural injury in the brain. There are no efficacious therapies available to treat Meth-induce neural injury. The present study intended to test the therapeutic potential of DL-3-n-butylphthalide (NBP), a chemical compound extracted originally from the seeds of Chinese Celery, in the amelioration and prevention of Meth-induced neural injury. Main methods Experiments were carried out on SH-SY5Y cells. Neuronal injury and apoptotic cell death were detected by MTT assay and analysis of nuclear morphology. Intracellular reactive oxygen species (ROS) was evaluated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay and protein expression levels of the apoptosis-related cleaved caspase-3, bcl2 and Bax were determined by western blotting. Key findings Treatment of SH-SY5Y cells with Meth induced cell injury and apoptosis. NBP attenuated Meth-associated cell injury and apoptosis via blockage of Meth-mediated upregulation of intracellular ROS production and inhibition of Meth-induced decrease of cleaved caspase-3/caspase-3 and Bcl-2/Bax ratios. Significance The results presented in this study indicate that NBP may have therapeutic benefits in the treatment of Meth-induced neuronal injury in the central nervous system.",
keywords = "DL-3-n-Butylphthalide, Methamphetamine, Neuroprotection, Neurotoxicity",
author = "Jun Zhao and Jianuo Liu and Enquan Xu and Yunlin Liu and Anmu Xie and Huangui Xiong",
year = "2016",
month = "11",
day = "15",
doi = "10.1016/j.lfs.2016.09.009",
language = "English (US)",
volume = "165",
pages = "16--20",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - DL-3-n-Butylphthalide attenuation of methamphetamine-induced neurotoxicity in SH-SY5Y neuroblastoma cells

AU - Zhao, Jun

AU - Liu, Jianuo

AU - Xu, Enquan

AU - Liu, Yunlin

AU - Xie, Anmu

AU - Xiong, Huangui

PY - 2016/11/15

Y1 - 2016/11/15

N2 - Aims Methamphetamine (Meth) abuse causes neural injury in the brain. There are no efficacious therapies available to treat Meth-induce neural injury. The present study intended to test the therapeutic potential of DL-3-n-butylphthalide (NBP), a chemical compound extracted originally from the seeds of Chinese Celery, in the amelioration and prevention of Meth-induced neural injury. Main methods Experiments were carried out on SH-SY5Y cells. Neuronal injury and apoptotic cell death were detected by MTT assay and analysis of nuclear morphology. Intracellular reactive oxygen species (ROS) was evaluated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay and protein expression levels of the apoptosis-related cleaved caspase-3, bcl2 and Bax were determined by western blotting. Key findings Treatment of SH-SY5Y cells with Meth induced cell injury and apoptosis. NBP attenuated Meth-associated cell injury and apoptosis via blockage of Meth-mediated upregulation of intracellular ROS production and inhibition of Meth-induced decrease of cleaved caspase-3/caspase-3 and Bcl-2/Bax ratios. Significance The results presented in this study indicate that NBP may have therapeutic benefits in the treatment of Meth-induced neuronal injury in the central nervous system.

AB - Aims Methamphetamine (Meth) abuse causes neural injury in the brain. There are no efficacious therapies available to treat Meth-induce neural injury. The present study intended to test the therapeutic potential of DL-3-n-butylphthalide (NBP), a chemical compound extracted originally from the seeds of Chinese Celery, in the amelioration and prevention of Meth-induced neural injury. Main methods Experiments were carried out on SH-SY5Y cells. Neuronal injury and apoptotic cell death were detected by MTT assay and analysis of nuclear morphology. Intracellular reactive oxygen species (ROS) was evaluated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay and protein expression levels of the apoptosis-related cleaved caspase-3, bcl2 and Bax were determined by western blotting. Key findings Treatment of SH-SY5Y cells with Meth induced cell injury and apoptosis. NBP attenuated Meth-associated cell injury and apoptosis via blockage of Meth-mediated upregulation of intracellular ROS production and inhibition of Meth-induced decrease of cleaved caspase-3/caspase-3 and Bcl-2/Bax ratios. Significance The results presented in this study indicate that NBP may have therapeutic benefits in the treatment of Meth-induced neuronal injury in the central nervous system.

KW - DL-3-n-Butylphthalide

KW - Methamphetamine

KW - Neuroprotection

KW - Neurotoxicity

UR - http://www.scopus.com/inward/record.url?scp=84988926601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84988926601&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2016.09.009

DO - 10.1016/j.lfs.2016.09.009

M3 - Article

VL - 165

SP - 16

EP - 20

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

ER -