Diurnal variations in intestinal barrier integrity and liver pathology in mice

Implications for alcohol binge

Robin M. Voigt, Christopher B. Forsyth, Maliha Shaikh, Lijuan Zhang, Shohreh Raeisi, Costica Aloman, Nailliw Z. Preite, Terrence Donohue, Louis Fogg, Ali Keshavarzian

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recent studies suggest that circadian rhythms regulate intestinal barrier integrity, but it is not clear whether there are daily variations in barrier integrity. This study investigated daily variations in intestinal barrier integrity, including whether there are differences in alcohol-induced intestinal barrier dysfunction after an alcohol binge at different times of day and whether this is associated with concurrent liver injury. C57BL6/J male mice were fed a standard chow diet, an alcohol-containing liquid diet, or an alcohol control diet for 4 wk. During week 5 (i.e., on days 43–45), mice received three once-daily gavages of alcohol (6 g/kg) or the control (phosphate-buffered saline) at the same time each day. Immediately after the binge on the second day, intestinal permeability was assessed. Four hours after the third and final binge, mice were eutha-nized and tissue samples collected. The results demonstrated diet-specific and outcome-specific effects of time, alcohol, and/or time by alcohol interaction. Specifically, the alcohol binge robustly influenced markers of intestinal barrier integrity, and liver markers were robustly influenced by time of day. Only intestinal permeability (i.e., sucra-lose) demonstrated a significant effect of time and also showed a binge by time interaction, suggesting that the time of the alcohol binge influences colonic permeability. NEW & NOTEWORTHY This study investigated daily variations in intestinal barrier integrity, including whether there are differences in alcohol-induced intestinal barrier dysfunction after an alcohol binge at different times of day and whether this is associated with concurrent liver injury. We conclude that 1) alcohol binge significantly impacted markers of intestinal permeability, 2) time of day significantly affected liver outcomes, and 3) the time of day influenced colonic permeability.

Original languageEnglish (US)
Pages (from-to)G131-G141
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume314
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Alcohols
Pathology
Liver
Permeability
Diet
Wounds and Injuries
Circadian Rhythm
Phosphates

Keywords

  • Alcohol binge
  • Circadian rhythms
  • Diurnal oscillations
  • Intestinal permeability
  • Intestine
  • Liver

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Diurnal variations in intestinal barrier integrity and liver pathology in mice : Implications for alcohol binge. / Voigt, Robin M.; Forsyth, Christopher B.; Shaikh, Maliha; Zhang, Lijuan; Raeisi, Shohreh; Aloman, Costica; Preite, Nailliw Z.; Donohue, Terrence; Fogg, Louis; Keshavarzian, Ali.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 314, No. 1, 01.01.2018, p. G131-G141.

Research output: Contribution to journalArticle

Voigt, Robin M. ; Forsyth, Christopher B. ; Shaikh, Maliha ; Zhang, Lijuan ; Raeisi, Shohreh ; Aloman, Costica ; Preite, Nailliw Z. ; Donohue, Terrence ; Fogg, Louis ; Keshavarzian, Ali. / Diurnal variations in intestinal barrier integrity and liver pathology in mice : Implications for alcohol binge. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2018 ; Vol. 314, No. 1. pp. G131-G141.
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