Distribution of [3H]AMPA binding sites in rat brain as determined by quantitative autoradiography

Daniel T. Monaghan, Deborah Yao, Carl W. Cotman

Research output: Contribution to journalArticle

195 Scopus citations

Abstract

Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) is a potent neuroexcitatory compound which acts at the quisqualate class of excitatory amino acid receptors. In this study we describe the pharmacological characteristics and anatomical distribution of [3H]AMPA binding sites in rat brain using quantitative autoradiography. These binding sites exhibit the appropriate pharmacological characteristics and are found in high concentrations in the hippocampus, cerebral cortex (especially layers I-III), induseum griseum, and dorsal lateral septum. Intermediate concentrations are found in the corpus striatum and deeper layers of cerebral cortex. Lower concentrations are found in the diencephalon, midbrain and brainstem. These results demonstrate that [3H]AMPA binding sites are found throughout the CNS and suggest brain regions which may use quisqualate receptors as glutamate neurotransmitter receptors.

Original languageEnglish (US)
Pages (from-to)160-164
Number of pages5
JournalBrain Research
Volume324
Issue number1
DOIs
Publication statusPublished - Dec 17 1984

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Keywords

  • amino-3-hydroxy-5-methyl-4-isoxazolepropionate
  • excitatory amino acids
  • glutamate receptors
  • quisqualate receptors
  • receptor autoradiography

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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