Distribution of kaposi sarcoma-associated herpesvirus/human herpesvirus 8 in maternal saliva and breast milk in Zambia: Implications for transmission

Brad P. Brayfield, Chipepo Kankasa, John T West, Jubra Muyanga, Ganapati Bhat, Winslow Klaskala, Charles D. Mitchell, Charles Wood

Research output: Contribution to journalArticle

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Abstract

Background. The seroprevalence of Kaposi sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) in sub-Saharan Africa suggests that multiple routes of transmission exist. In the present study, we examined 2 possible routes of mother-to-child transmission, through breast milk and saliva, during the first 6 months after delivery. Methods. The prevalence of HHV-8 DNA in the breast-milk cells (n = 75), milk supernatant (n = 56), colostrum (n = 2), and saliva cells (n = 65) of HHV-8-seropositive mothers who recently gave birth was examined. Polymerase chain reaction analysis was performed for the detection of HHV-8 in cross-sectional samples isolated at 2, 4, and 6 months after delivery. Results. None of the 75 breast-milk samples but 2 of the colostrum samples that were analyzed contained HHV-8 DNA at a limit of detection of ∼1 HHV-8 copy/104 cellular genomes, whereas Epstein-Barr virus DNA and HIV-1 DNA were detected in 16 and 22 samples, respectively. Analysis of 65 saliva cell samples, which were obtained from mothers who also provided milk samples, revealed that 19 of the samples had detectable HHV-8 DNA. Viral DNA was found at all time points, but the presence of viral DNA in saliva was independent of maternal HIV-1 serostatus (χ2 = 0.33; P = .57). Conclusions. Our findings demonstrate the lack of HHV-8 DNA in the breast milk of seropositive mothers, and they suggest that contact with breast milk is not a likely source of horizontal transmission of virus to infants in sub-Saharan Africa.

Original languageEnglish (US)
Pages (from-to)2260-2270
Number of pages11
JournalJournal of Infectious Diseases
Volume189
Issue number12
DOIs
StatePublished - Jun 15 2004

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Zambia
Human Herpesvirus 8
Human Milk
Saliva
Mothers
DNA
Colostrum
Africa South of the Sahara
Viral DNA
HIV-1
Milk
Seroepidemiologic Studies
Human Herpesvirus 4
Limit of Detection
Parturition
Genome
Viruses
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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Distribution of kaposi sarcoma-associated herpesvirus/human herpesvirus 8 in maternal saliva and breast milk in Zambia : Implications for transmission. / Brayfield, Brad P.; Kankasa, Chipepo; West, John T; Muyanga, Jubra; Bhat, Ganapati; Klaskala, Winslow; Mitchell, Charles D.; Wood, Charles.

In: Journal of Infectious Diseases, Vol. 189, No. 12, 15.06.2004, p. 2260-2270.

Research output: Contribution to journalArticle

Brayfield, Brad P. ; Kankasa, Chipepo ; West, John T ; Muyanga, Jubra ; Bhat, Ganapati ; Klaskala, Winslow ; Mitchell, Charles D. ; Wood, Charles. / Distribution of kaposi sarcoma-associated herpesvirus/human herpesvirus 8 in maternal saliva and breast milk in Zambia : Implications for transmission. In: Journal of Infectious Diseases. 2004 ; Vol. 189, No. 12. pp. 2260-2270.
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abstract = "Background. The seroprevalence of Kaposi sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) in sub-Saharan Africa suggests that multiple routes of transmission exist. In the present study, we examined 2 possible routes of mother-to-child transmission, through breast milk and saliva, during the first 6 months after delivery. Methods. The prevalence of HHV-8 DNA in the breast-milk cells (n = 75), milk supernatant (n = 56), colostrum (n = 2), and saliva cells (n = 65) of HHV-8-seropositive mothers who recently gave birth was examined. Polymerase chain reaction analysis was performed for the detection of HHV-8 in cross-sectional samples isolated at 2, 4, and 6 months after delivery. Results. None of the 75 breast-milk samples but 2 of the colostrum samples that were analyzed contained HHV-8 DNA at a limit of detection of ∼1 HHV-8 copy/104 cellular genomes, whereas Epstein-Barr virus DNA and HIV-1 DNA were detected in 16 and 22 samples, respectively. Analysis of 65 saliva cell samples, which were obtained from mothers who also provided milk samples, revealed that 19 of the samples had detectable HHV-8 DNA. Viral DNA was found at all time points, but the presence of viral DNA in saliva was independent of maternal HIV-1 serostatus (χ2 = 0.33; P = .57). Conclusions. Our findings demonstrate the lack of HHV-8 DNA in the breast milk of seropositive mothers, and they suggest that contact with breast milk is not a likely source of horizontal transmission of virus to infants in sub-Saharan Africa.",
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T2 - Implications for transmission

AU - Brayfield, Brad P.

AU - Kankasa, Chipepo

AU - West, John T

AU - Muyanga, Jubra

AU - Bhat, Ganapati

AU - Klaskala, Winslow

AU - Mitchell, Charles D.

AU - Wood, Charles

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N2 - Background. The seroprevalence of Kaposi sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) in sub-Saharan Africa suggests that multiple routes of transmission exist. In the present study, we examined 2 possible routes of mother-to-child transmission, through breast milk and saliva, during the first 6 months after delivery. Methods. The prevalence of HHV-8 DNA in the breast-milk cells (n = 75), milk supernatant (n = 56), colostrum (n = 2), and saliva cells (n = 65) of HHV-8-seropositive mothers who recently gave birth was examined. Polymerase chain reaction analysis was performed for the detection of HHV-8 in cross-sectional samples isolated at 2, 4, and 6 months after delivery. Results. None of the 75 breast-milk samples but 2 of the colostrum samples that were analyzed contained HHV-8 DNA at a limit of detection of ∼1 HHV-8 copy/104 cellular genomes, whereas Epstein-Barr virus DNA and HIV-1 DNA were detected in 16 and 22 samples, respectively. Analysis of 65 saliva cell samples, which were obtained from mothers who also provided milk samples, revealed that 19 of the samples had detectable HHV-8 DNA. Viral DNA was found at all time points, but the presence of viral DNA in saliva was independent of maternal HIV-1 serostatus (χ2 = 0.33; P = .57). Conclusions. Our findings demonstrate the lack of HHV-8 DNA in the breast milk of seropositive mothers, and they suggest that contact with breast milk is not a likely source of horizontal transmission of virus to infants in sub-Saharan Africa.

AB - Background. The seroprevalence of Kaposi sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) in sub-Saharan Africa suggests that multiple routes of transmission exist. In the present study, we examined 2 possible routes of mother-to-child transmission, through breast milk and saliva, during the first 6 months after delivery. Methods. The prevalence of HHV-8 DNA in the breast-milk cells (n = 75), milk supernatant (n = 56), colostrum (n = 2), and saliva cells (n = 65) of HHV-8-seropositive mothers who recently gave birth was examined. Polymerase chain reaction analysis was performed for the detection of HHV-8 in cross-sectional samples isolated at 2, 4, and 6 months after delivery. Results. None of the 75 breast-milk samples but 2 of the colostrum samples that were analyzed contained HHV-8 DNA at a limit of detection of ∼1 HHV-8 copy/104 cellular genomes, whereas Epstein-Barr virus DNA and HIV-1 DNA were detected in 16 and 22 samples, respectively. Analysis of 65 saliva cell samples, which were obtained from mothers who also provided milk samples, revealed that 19 of the samples had detectable HHV-8 DNA. Viral DNA was found at all time points, but the presence of viral DNA in saliva was independent of maternal HIV-1 serostatus (χ2 = 0.33; P = .57). Conclusions. Our findings demonstrate the lack of HHV-8 DNA in the breast milk of seropositive mothers, and they suggest that contact with breast milk is not a likely source of horizontal transmission of virus to infants in sub-Saharan Africa.

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