Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs

Alan R Doster, Sakthivel Subramaniam, Ji Young Yhee, Byung Joon Kwon, Chi Ho Yu, So Young Kwon, Fernando A. Osorio, Jung Hyang Sur

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Distribution and characterization of interlukin-10 (IL-10)-secreting cells in lymphoid tissues of pigs naturally infected with porcine circovirus type 2 (PCV2) were evaluated in accordance with PCV2 antigen detection. After screening a total of 56 pigs showing the symptoms of postweaning multisystemic wasting syndrome (PMWS), 15 pigs were PCV2 positive and 5 pigs, which showed stronger positive signals over multiples tissues were further investigated. This study showed that in PCV2-infected lymphoid tissues, particularly mandibular lymph node, spleen and tonsil, IL-10 expression was mainly localized in T-cell rich areas but rarely in B cell rich areas. IL-10 was highly expressed in bystander cells but rarely in PCV2-infected cells. Elevated IL-10 expression was predominantly associated with T cells, but rarely with B cells or with macrophages. The results of this study provide evidence for the role of IL-10 in chronic PCV2 infection and its relation to PCV2 antigen in affected tissues. Constantly elevated levels of IL-10 lead to immunosuppression in persistent and chronic viral infections. The increased IL-10 expression observed in PCV2 infection in this study suggests that IL-10-mediated immunosuppression may play an important role in the pathogenesis and maintenance of naturally occurring PCV2 infection.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalJournal of Veterinary Science
Volume11
Issue number3
DOIs
StatePublished - Sep 21 2010

Fingerprint

Circovirus
Porcine circovirus-2
Lymphoid Tissue
Swine
swine
Circoviridae Infections
cells
immunosuppression
infection
Immunosuppression
B-lymphocytes
B-Lymphocytes
T-lymphocytes
Wasting Syndrome
postweaning multisystemic wasting syndrome
T-Lymphocytes
tissues
Antigens
antigen detection
tonsils

Keywords

  • B-cells
  • Immunohistochemistry
  • Interlukin-10
  • Porcine circovirus type 2
  • T-cells

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs. / Doster, Alan R; Subramaniam, Sakthivel; Yhee, Ji Young; Kwon, Byung Joon; Yu, Chi Ho; Kwon, So Young; Osorio, Fernando A.; Sur, Jung Hyang.

In: Journal of Veterinary Science, Vol. 11, No. 3, 21.09.2010, p. 177-183.

Research output: Contribution to journalArticle

Doster, Alan R ; Subramaniam, Sakthivel ; Yhee, Ji Young ; Kwon, Byung Joon ; Yu, Chi Ho ; Kwon, So Young ; Osorio, Fernando A. ; Sur, Jung Hyang. / Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs. In: Journal of Veterinary Science. 2010 ; Vol. 11, No. 3. pp. 177-183.
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