Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans

Shaojie Li, Liangcheng Du, Gary Yuen, Steven D. Harris

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

In filamentous fungi, the stabilization of a polarity axis is likely to be a pivotal event underlying the emergence of a germ tube from a germinating spore. Recent results implicate the polarisome in this process and also suggest that it requires localized membrane organization. Here, we employ a chemical genetic approach to demonstrate that ceramide synthesis is necessary for the formation of a stable polarity axis in the model fungus Aspergillus nidulans. We demonstrate that a novel compound (HSAF) produced by a bacterial biocontrol agent disrupts polarized growth and leads to loss of membrane organization and formin localization at hyphal tips. We show that BarA, a putative acyl-CoA-dependent ceramide synthase that is unique to filamentous fungi mediates the effects of HSAF. Moreover, A. nidulans possesses a second likely ceramide synthase that is essential and also regulates hyphal morphogenesis. Our results suggest that filamentous fungi possess distinct pools of ceramide that make independent contributions to polarized hyphal growth, perhaps through the formation of specialized lipid microdomains that regulate organization of the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)1218-1227
Number of pages10
JournalMolecular biology of the cell
Volume17
Issue number3
DOIs
StatePublished - Mar 1 2006

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Aspergillus nidulans
Fungi
Ceramides
Growth
Acyl Coenzyme A
Membranes
Spores
Cytoskeleton
Morphogenesis
Lipids
dihydroceramide desaturase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans. / Li, Shaojie; Du, Liangcheng; Yuen, Gary; Harris, Steven D.

In: Molecular biology of the cell, Vol. 17, No. 3, 01.03.2006, p. 1218-1227.

Research output: Contribution to journalArticle

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