Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs

Martin L. Tomov, Zachary T. Olmsted, Haluk Dogan, Eda Gongorurler, Maria Tsompana, Hasan H Otu, Michael Buck, Eun Ah Chang, Jose Cibelli, Janet L. Paluh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The realization of personalized medicine through human induced pluripotent stem cell (iPSC) technology can be advanced by transcriptomics, epigenomics, and bioinformatics that inform on genetic pathways directing tissue development and function. When possible, population diversity should be included in new studies as resources become available. Previously we derived replicate iPSC lines of African American, Hispanic-Latino and Asian self-designated ethnically diverse (ED) origins with normal karyotype, verified teratoma formation, pluripotency biomarkers, and tri-lineage in vitro commitment. Here we perform bioinformatics of RNA-Seq and ChIP-seq pluripotency data sets for two replicate Asian and Hispanic-Latino ED-iPSC lines that reveal differences in generation of contractile cardiomyocytes but similar and robust differentiation to multiple neural, pancreatic, and smooth muscle cell types. We identify shared and distinct genes and contributing pathways in the replicate ED-iPSC lines to enhance our ability to understand how reprogramming to iPSC impacts genes and pathways contributing to cardiomyocyte contractility potential.

Original languageEnglish (US)
Article number37637
JournalScientific Reports
Volume6
DOIs
StatePublished - Dec 5 2016

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Induced Pluripotent Stem Cells
Cardiac Myocytes
Hispanic Americans
Computational Biology
Cell Line
Precision Medicine
Teratoma
Karyotype
Epigenomics
African Americans
Genes
Smooth Muscle Myocytes
Biomarkers
RNA
Technology
Population

ASJC Scopus subject areas

  • General

Cite this

Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs. / Tomov, Martin L.; Olmsted, Zachary T.; Dogan, Haluk; Gongorurler, Eda; Tsompana, Maria; Otu, Hasan H; Buck, Michael; Chang, Eun Ah; Cibelli, Jose; Paluh, Janet L.

In: Scientific Reports, Vol. 6, 37637, 05.12.2016.

Research output: Contribution to journalArticle

Tomov, ML, Olmsted, ZT, Dogan, H, Gongorurler, E, Tsompana, M, Otu, HH, Buck, M, Chang, EA, Cibelli, J & Paluh, JL 2016, 'Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs', Scientific Reports, vol. 6, 37637. https://doi.org/10.1038/srep37637
Tomov, Martin L. ; Olmsted, Zachary T. ; Dogan, Haluk ; Gongorurler, Eda ; Tsompana, Maria ; Otu, Hasan H ; Buck, Michael ; Chang, Eun Ah ; Cibelli, Jose ; Paluh, Janet L. / Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs. In: Scientific Reports. 2016 ; Vol. 6.
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