Dissimilatory arsenate reductase activity and arsenate-respiring bacteria in bovine rumen fluid, hamster feces, and the termite hindgut

Mitchell J. Herbel, Jodi Switzer Blum, Shelley E. Hoeft, Samuel Monroe Cohen, Lora L Arnold, Joy Lisak, John F. Stolz, Ronald S. Oremland

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Bovine rumen fluid and slurried hamster feces completely reduced millimolar levels of arsenate to arsenite upon incubation under anoxic conditions. This activity was strongly inhibited by autoclaving or aerobic conditions, and partially inhibited by tungstate or chloramphenicol. The rate of arsenate reduction was faster in feces from a population of arsenate-watered (100 ppm) hamsters compared to a control group watered without arsenate. Using radioisotope methods, arsenate reductase activity in hamster feces was also detected at very low concentrations of added arsenate (∼10 μM). Bacterial cultures were isolated from these materials, as well as from the termite hindgut, that grew using H2 as their electron donor, acetate as their carbon source, and arsenate as their respiratory electron acceptor. The three cultures aligned phylogenetically either with well-established enteric bacteria, or with an organism associated with feedlot fecal wastes. Because arsenite is transported across the gut epithelium more readily than arsenate, microbial dissimilatory reduction of arsenate in the gut may promote the body's absorption of arsenic and hence potentiate its toxicity.

Original languageEnglish (US)
Pages (from-to)59-67
Number of pages9
JournalFEMS Microbiology Ecology
Volume41
Issue number1
DOIs
StatePublished - Jul 3 2002

Fingerprint

Arsenate Reductases
Isoptera
Rumen
termite
arsenate
Feces
feces
Cricetinae
Bacteria
bacterium
fluid
arsenite
Electrons
arsenic acid
bovine
electron
Arsenic
Enterobacteriaceae
Chloramphenicol
Radioisotopes

Keywords

  • Anaerobe
  • Arsenic
  • GI Tract

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Dissimilatory arsenate reductase activity and arsenate-respiring bacteria in bovine rumen fluid, hamster feces, and the termite hindgut. / Herbel, Mitchell J.; Switzer Blum, Jodi; Hoeft, Shelley E.; Cohen, Samuel Monroe; Arnold, Lora L; Lisak, Joy; Stolz, John F.; Oremland, Ronald S.

In: FEMS Microbiology Ecology, Vol. 41, No. 1, 03.07.2002, p. 59-67.

Research output: Contribution to journalArticle

Herbel, Mitchell J. ; Switzer Blum, Jodi ; Hoeft, Shelley E. ; Cohen, Samuel Monroe ; Arnold, Lora L ; Lisak, Joy ; Stolz, John F. ; Oremland, Ronald S. / Dissimilatory arsenate reductase activity and arsenate-respiring bacteria in bovine rumen fluid, hamster feces, and the termite hindgut. In: FEMS Microbiology Ecology. 2002 ; Vol. 41, No. 1. pp. 59-67.
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