Dissection of a QTL hotspot on mouse distal chromosome 1 that modulates neurobehavioral phenotypes and gene expression

Khyobeni Mozhui, Daniel C. Ciobanu, Thomas Schikorski, Xusheng Wang, Lu Lu, Robert W. Williams

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A remarkably diverse set of traits maps to a region on mouse distal chromosome 1 (Chr 1) that corresponds to human Chr 1q21-q23. This region is highly enriched in quantitative trait loci (QTLs) that control neural and behavioral phenotypes, including motor behavior, escape latency, emotionality, seizure susceptibility (Szs1), and responses to ethanol, caffeine, pentobarbital, and haloperidol. This region also controls the expression of a remarkably large number of genes, including genes that are associated with some of the classical traits that map to distal Chr 1 (e.g., seizure susceptibility). Here, we ask whether this QTL-rich region on Chr 1 (Qrr1) consists of a single master locus or a mixture of linked, but functionally unrelated, QTLs. To answer this question and to evaluate candidate genes, we generated and analyzed several gene expression, haplotype, and sequence datasets. We exploited six complementary mouse crosses, and combed through 18 expression datasets to determine class membership of genes modulated by Qrr1. Qrr1 can be broadly divided into a proximal part (Qrr1p) and a distal part (Qrr1d), each associated with the expression of distinct subsets of genes. Qrr1d controls RNA metabolism and protein synthesis, including the expression of ∼20 aminoacyl-tRNA synthetases. Qrr1d contains a tRNA cluster, and this is a functionally pertinent candidate for the tRNA synthetases. Rgs7 and Fmn2 are other strong candidates in Qrr1d. FMN2 protein has pronounced expression in neurons, including in the dendrites, and deletion of Fmn2 had a strong effect on the expression of few genes modulated by Qrr1d. Our analysis revealed a highly complex gene expression regulatory interval in Qrr1, composed of multiple loci modulating the expression of functionally cognate sets of genes.

Original languageEnglish (US)
Article numbere1000260
JournalPLoS genetics
Volume4
Issue number11
DOIs
StatePublished - Nov 1 2008

Fingerprint

Chromosomes, Human, Pair 1
Quantitative Trait Loci
dissection
gene expression
phenotype
Dissection
chromosome
quantitative trait loci
chromosomes
Phenotype
Gene Expression
gene
mice
Genes
Amino Acyl-tRNA Synthetases
genes
aminoacyl tRNA ligases
seizures
Seizures
haloperidol

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Dissection of a QTL hotspot on mouse distal chromosome 1 that modulates neurobehavioral phenotypes and gene expression. / Mozhui, Khyobeni; Ciobanu, Daniel C.; Schikorski, Thomas; Wang, Xusheng; Lu, Lu; Williams, Robert W.

In: PLoS genetics, Vol. 4, No. 11, e1000260, 01.11.2008.

Research output: Contribution to journalArticle

Mozhui, Khyobeni ; Ciobanu, Daniel C. ; Schikorski, Thomas ; Wang, Xusheng ; Lu, Lu ; Williams, Robert W. / Dissection of a QTL hotspot on mouse distal chromosome 1 that modulates neurobehavioral phenotypes and gene expression. In: PLoS genetics. 2008 ; Vol. 4, No. 11.
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