Disruption of the olfactory placode and brain conditioned medium increase the number of LHRH immunostained neurons in explants

M. R L Maas, Robert B Norgren

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Abstract

The olfactory placode gives rise to both olfactory receptor neurons, which remain as a component of the peripheral nervous system, and to luteinizing hormone-releasing hormone (LHRH) neurons, which migrate to the central nervous system. In this study, we used chick olfactory placode explants to ask several questions regarding LHRH neuronal differentiation. We found that explants of ectoderm from the fronto-nasal region of embryos as early as Hamilton and Hamburger (HH) stage 12 gave rise to LHRH neurons, that explants from all regions of the olfactory placode were able to generate LHRH neurons, that both brain conditioned medium and disruption of the olfactory placode increase the number of LHRH neurons observed in explants, and that the combination of these two manipulations results in the production of more LHRH neurons than either treatment alone. We conclude that LHRH neurons originate in the olfactory epithelium and that some of the same factors which influence olfactory receptor neuron development also affect LHRH neuronal development. (C) 2000 Harcourt Publishers Ltd.

Original languageEnglish (US)
Pages (from-to)216-222
Number of pages7
JournalTissue and Cell
Volume32
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

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Keywords

  • Development
  • Differentiation
  • GnRH
  • Olfactory epithelium

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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