Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis

Henrik Tjellström, Merissa Strawsine, Jillian Silva, Edgar B. Cahoon, John B. Ohlrogge

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e.g.; C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8:0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids.

Original languageEnglish (US)
Pages (from-to)936-942
Number of pages7
JournalFEBS Letters
Volume587
Issue number7
DOIs
StatePublished - Apr 2 2013

Fingerprint

Plastids
Arabidopsis
Seed
Seeds
Fatty Acids
Cuphea
Chemical activation
Genetically Modified Plants
Chain length
Demonstrations
long-chain-fatty-acid-(acyl-carrier-protein) ligase
Enzymes

Keywords

  • AAE15/16
  • Acyl chain activating enzyme
  • Cuphea
  • Medium chain fatty acid
  • Thioesterase
  • Triacylglycerol

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis. / Tjellström, Henrik; Strawsine, Merissa; Silva, Jillian; Cahoon, Edgar B.; Ohlrogge, John B.

In: FEBS Letters, Vol. 587, No. 7, 02.04.2013, p. 936-942.

Research output: Contribution to journalArticle

Tjellström, Henrik ; Strawsine, Merissa ; Silva, Jillian ; Cahoon, Edgar B. ; Ohlrogge, John B. / Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis. In: FEBS Letters. 2013 ; Vol. 587, No. 7. pp. 936-942.
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