Disposable hydrophobic surface on MALDI targets for enhancing MS and MS/MS data of peptides

Rebekah L. Gundry, Roy Edward, Thomas P. Kole, Chris Sutton, Robert J. Cotter

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Automated analyses in MALDI MS are complicated by the uneven distribution of analyte over the sample spot, resulting in areas of analyte localization, or "sweet spots". Hence, the ability to concentrate and localize samples is advantageous, especially for automated studies involving low concentrations of analyte. A method for rapidly creating a removable and affordable hydrophobic surface that is free from memory effect is presented. The potential for such compounds to serve as a practical coating for MALDI targets is examined. An example compound with a complete methodology is shown to increase sample homogeneity, peak intensity, and resolution when used for peptide mixtures with CHCA and DHB.

Original languageEnglish (US)
Pages (from-to)6609-6617
Number of pages9
JournalAnalytical chemistry
Volume77
Issue number20
DOIs
StatePublished - Oct 15 2005

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ASJC Scopus subject areas

  • Analytical Chemistry

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