Discovery of novel NGS-mined microsatellite markers and an exploratory analysis of genetic differentiation between two Western Atlantic populations of Cardisoma guanhumi Latreille, 1825 (Decapoda: Brachyura: Gecarcinidae)

Sulimary O. Gomes, Isis G.B. Souza, Michelli F. Santos, Geice R. Silva, Marc Albrecht, Alan S. McKinley, Paul Bentzen, Fabio M. Diniz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The blue land crab Cardisoma guanhumi Latreille, 1825 is a highly valued food resource widespread in tropical and subtropical regions of the west Atlantic region. Current overharvesting, however, has played a significant role in stock decline. We describe the development of 12 new microsatellite markers, by next-generation sequencing, for evaluating genetic diversity and population structure across the geographical range of the species. A glimpse into the genetic variation in the Caribbean is also provided. The highly informative nature of these markers was also given by the mean estimate values for allelic richness (AR), gene diversity, and polymorphic information content (PIC); 3.8, 0.593, 0.518 in Florida and 2.9, 0.450, 0.385 in Puerto Rico. A preliminary genetic diversity analysis revealed high population differentiation between both locations (FST = 0.310 and DEST = 0.526). PCoA and Bayesian clustering provisionally detected two genetically distinct groups based on the two predefined populations from the Caribbean.

Original languageEnglish (US)
Pages (from-to)181-185
Number of pages5
JournalJournal of Crustacean Biology
Volume39
Issue number2
DOIs
StatePublished - Apr 5 2019

Fingerprint

genetic differentiation
Decapoda
microsatellite repeats
genetic variation
subtropical region
tropical region
population structure
crab
Puerto Rico
subtropics
food
gene
tropics
crabs
resource
genetic diversity
analysis
marker
genes
land

Keywords

  • Blue land crab
  • Florida
  • NGS technology
  • Puerto Rico
  • conservation
  • molecular markers
  • population genetics

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Discovery of novel NGS-mined microsatellite markers and an exploratory analysis of genetic differentiation between two Western Atlantic populations of Cardisoma guanhumi Latreille, 1825 (Decapoda : Brachyura: Gecarcinidae). / Gomes, Sulimary O.; Souza, Isis G.B.; Santos, Michelli F.; Silva, Geice R.; Albrecht, Marc; McKinley, Alan S.; Bentzen, Paul; Diniz, Fabio M.

In: Journal of Crustacean Biology, Vol. 39, No. 2, 05.04.2019, p. 181-185.

Research output: Contribution to journalArticle

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