Discovery of a 240 million year old nematode parasite egg in a cynodont coprolite sheds light on the early origin of pinworms in vertebrates

Jean Pierre Hugot, Scott L. Gardner, Victor Borba, Priscilla Araujo, Daniela Leles, Átila Augusto Stock Da-Rosa, Juliana Dutra, Luiz Fernando Ferreira, Adauto Araújo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: We report the discovery of a nematode parasite egg (Nemata: Oxyurida) from a coprolite closely associated with the remains of several species of Cynodontia, dated to 240 million years old. This finding is particularly significant because this is the oldest record of an oxyurid nematode yet discovered, and because the cynodonts are considered a stem-group of the mammals. Methods: We extracted material from a fully mineralized coprolite by both scraping the surface, and removing fragments from its interior with clean dental instruments used a single time. A single drop of glycerol from a new vial was added as a clearing reagent. Each slide was sealed with wax and examined with an optical microscope at 100× to 400× magnification. Results: From one coprolite, 550 slides were examined; from 275 of these slides, sediment was examined that was scraped from the surface of the coprolite, and from the other 275 slides, material was examined that was extracted from the interior of the coprolite. All microscopic structures encountered were photographed, measured, and identified when possible. Conclusions: From the coprolite examined, we discovered an egg representing a new species of pinworm that, based on the egg structure, clearly places it in the family Heteroxynematidae. Nematodes of the order Oxyurida have very constrained life-histories, occurring only in animals that are not strictly carnivorous and also ingest large amounts of plant material. This fact enabled us to determine which species of cynodont, from several collected at the site in Brazil, are most likely the depositors of the coprolite, and therefore were the putative host of the parasite.

Original languageEnglish (US)
Article number486
JournalParasites and Vectors
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2014

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Enterobius
Oxyurida
Ovum
Vertebrates
Parasites
Dental Instruments
Waxes
Glycerol
Brazil
Mammals

Keywords

  • Brazil
  • Coprolite
  • Cynodont
  • Haplodiploid
  • Heteroxynematidae
  • Oxyurida
  • Paleoparasitology
  • Pinworm
  • Upper Triassic

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Discovery of a 240 million year old nematode parasite egg in a cynodont coprolite sheds light on the early origin of pinworms in vertebrates. / Hugot, Jean Pierre; Gardner, Scott L.; Borba, Victor; Araujo, Priscilla; Leles, Daniela; Da-Rosa, Átila Augusto Stock; Dutra, Juliana; Ferreira, Luiz Fernando; Araújo, Adauto.

In: Parasites and Vectors, Vol. 7, No. 1, 486, 01.01.2014.

Research output: Contribution to journalArticle

Hugot, Jean Pierre ; Gardner, Scott L. ; Borba, Victor ; Araujo, Priscilla ; Leles, Daniela ; Da-Rosa, Átila Augusto Stock ; Dutra, Juliana ; Ferreira, Luiz Fernando ; Araújo, Adauto. / Discovery of a 240 million year old nematode parasite egg in a cynodont coprolite sheds light on the early origin of pinworms in vertebrates. In: Parasites and Vectors. 2014 ; Vol. 7, No. 1.
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abstract = "Background: We report the discovery of a nematode parasite egg (Nemata: Oxyurida) from a coprolite closely associated with the remains of several species of Cynodontia, dated to 240 million years old. This finding is particularly significant because this is the oldest record of an oxyurid nematode yet discovered, and because the cynodonts are considered a stem-group of the mammals. Methods: We extracted material from a fully mineralized coprolite by both scraping the surface, and removing fragments from its interior with clean dental instruments used a single time. A single drop of glycerol from a new vial was added as a clearing reagent. Each slide was sealed with wax and examined with an optical microscope at 100× to 400× magnification. Results: From one coprolite, 550 slides were examined; from 275 of these slides, sediment was examined that was scraped from the surface of the coprolite, and from the other 275 slides, material was examined that was extracted from the interior of the coprolite. All microscopic structures encountered were photographed, measured, and identified when possible. Conclusions: From the coprolite examined, we discovered an egg representing a new species of pinworm that, based on the egg structure, clearly places it in the family Heteroxynematidae. Nematodes of the order Oxyurida have very constrained life-histories, occurring only in animals that are not strictly carnivorous and also ingest large amounts of plant material. This fact enabled us to determine which species of cynodont, from several collected at the site in Brazil, are most likely the depositors of the coprolite, and therefore were the putative host of the parasite.",
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AU - Araujo, Priscilla

AU - Leles, Daniela

AU - Da-Rosa, Átila Augusto Stock

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AU - Ferreira, Luiz Fernando

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