Discordant memory B cell and circulating anti-Env antibody responses in HIV-1 infection

Yongjun Guan, Mohammad M. Sajadi, Roberta Kamin-Lewis, Timothy R. Fouts, Anthony Dimitrov, Zhixin Zhang, Robert R. Redfield, Anthony L. DeVico, Robert C. Gallo, George K. Lewis

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Long-lived memory B cells (BMem) provide an archive of historic Ab responses. By contrast, circulating Abs typically decline once the immunogen is cleared. Consequently, circulating Abs can underestimate the nature of cognate humoral immunity. On the other hand, the BMem pool should provide a comprehensive picture of Ab specificities that arise over the entire course of infection. To test this hypothesis, we compared circulating Ab and BMem from natural virus suppressors who control HIV-1 without therapy and maintain a relatively intact immune system. We found high frequencies of BMem specific for the conserved neutralizing CD4 induced or CD4 binding site epitopes of gp120, whereas low Ab titers to these determinants were detected in contemporaneous plasma. These data suggest that plasma Ab repertoires can underestimate the breadth of humoral immunity, and analyses of BMem should be included in studies correlating Ab specificity with protective immunity to HIV-1.

Original languageEnglish (US)
Pages (from-to)3952-3957
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number10
DOIs
Publication statusPublished - Mar 10 2009

    Fingerprint

Keywords

  • CD4bs antibody
  • CD4i antibody
  • Elite controller
  • Human monoclonal antibody
  • Natural viral suppressors

ASJC Scopus subject areas

  • General

Cite this