Discontinuous fatty acid elongation yields hydroxylated seed oil with improved function

Xiangjun Li, Alicen M. Teitgen, Asghar Shirani, Juan Ling, Lucas Busta, Rebecca E. Cahoon, Wei Zhang, Zaiyun Li, Kent D. Chapman, Diana Berman, Chunyu Zhang, Robert E. Minto, Edgar B. Cahoon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The biosynthesis of ‘unusual’ fatty acids with structures that deviate from the common C16 and C18 fatty acids has evolved numerous times in the plant kingdom. Characterization of unusual fatty acid biosynthesis has enabled increased understanding of enzyme substrate properties, metabolic plasticity and oil functionality. Here, we report the identification of a novel pathway for hydroxy fatty acid biosynthesis based on the serendipitous discovery of two C24 fatty acids containing hydroxyl groups at the 7 and 18 carbon atoms as major components of the seed oil of Orychophragmus violaceus, a China-native Brassicaceae. Biochemical and genetic evidence are presented for premature or ‘discontinuous’ elongation of a 3-OH intermediate by a divergent 3-ketoacyl-CoA (coenzyme A) synthase during a chain extension cycle as the origin of the 7-OH group of the dihydroxy fatty acids. Tribology studies revealed superior high-temperature lubricant properties for O. violaceus seed oil compared to castor oil, a high-performance vegetable oil lubricant. These findings provide a direct pathway for designing a new class of environmentally friendly lubricants and unveil the potential of O. violaceus as a new industrial oilseed crop.

Original languageEnglish (US)
Pages (from-to)711-720
Number of pages10
JournalNature Plants
Volume4
Issue number9
DOIs
StatePublished - Sep 1 2018

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seed oils
lubricants
fatty acids
biosynthesis
hydroxy fatty acids
castor oil
oilseed crops
coenzyme A
enzyme substrates
Brassicaceae
vegetable oil
oils
China
carbon
Orychophragmus violaceus
temperature

ASJC Scopus subject areas

  • Plant Science

Cite this

Discontinuous fatty acid elongation yields hydroxylated seed oil with improved function. / Li, Xiangjun; Teitgen, Alicen M.; Shirani, Asghar; Ling, Juan; Busta, Lucas; Cahoon, Rebecca E.; Zhang, Wei; Li, Zaiyun; Chapman, Kent D.; Berman, Diana; Zhang, Chunyu; Minto, Robert E.; Cahoon, Edgar B.

In: Nature Plants, Vol. 4, No. 9, 01.09.2018, p. 711-720.

Research output: Contribution to journalArticle

Li, X, Teitgen, AM, Shirani, A, Ling, J, Busta, L, Cahoon, RE, Zhang, W, Li, Z, Chapman, KD, Berman, D, Zhang, C, Minto, RE & Cahoon, EB 2018, 'Discontinuous fatty acid elongation yields hydroxylated seed oil with improved function', Nature Plants, vol. 4, no. 9, pp. 711-720. https://doi.org/10.1038/s41477-018-0225-7
Li, Xiangjun ; Teitgen, Alicen M. ; Shirani, Asghar ; Ling, Juan ; Busta, Lucas ; Cahoon, Rebecca E. ; Zhang, Wei ; Li, Zaiyun ; Chapman, Kent D. ; Berman, Diana ; Zhang, Chunyu ; Minto, Robert E. ; Cahoon, Edgar B. / Discontinuous fatty acid elongation yields hydroxylated seed oil with improved function. In: Nature Plants. 2018 ; Vol. 4, No. 9. pp. 711-720.
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