Direct regulation of extracellular chitinase production by the transcription factor LeClp in Lysobacter enzymogenes OH11

Huiyong Xu, Hongfu Chen, Yuemao Shen, Liangcheng Du, Shan Ho Chou, Hongxia Liu, Guoliang Qian, Fengquan Liu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Lysobacter enzymogenes is a gram-negative bacterial biological control agent that produces abundant extracellular enzymes capable of degrading the cell walls of fungal pathogens. In strain OH11, an isolate from China, the global regulator LeClp controls the production of extracellular chitinase by regulating the transcription of the chitinase-encoding gene chiA. Using a combination of bioinformatic, genetic, and biochemical methods, we show that LeClp regulates chiA transcription by directly binding to the chiA promoter region. Although LeClp appears to be important in this role, it is not the sole regulator of chiA transcription. Furthermore, the sequence analysis of putative LeClp binding sites indicated that the LeClp homolog could be involved in the regulation of extracellular chitinase production in diverse Lysobacter spp. by a mechanism similar to that in L. enzymogenes. Our findings present new insights into the molecular mechanism of LeClp in controlling extracellular chitinase activity, providing a fundamental road to elucidate how LeClp regulates the production of other extracellular lytic enzymes in L. enzymogenes.

Original languageEnglish (US)
Pages (from-to)971-977
Number of pages7
JournalPhytopathology
Volume106
Issue number9
DOIs
StatePublished - Sep 2016

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Lysobacter enzymogenes
chitinase
transcription factors
Lysobacter
transcription (genetics)
enzymes
bioinformatics
biological control agents
roads
binding sites
sequence analysis
promoter regions
cell walls
China
pathogens
genes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Direct regulation of extracellular chitinase production by the transcription factor LeClp in Lysobacter enzymogenes OH11. / Xu, Huiyong; Chen, Hongfu; Shen, Yuemao; Du, Liangcheng; Chou, Shan Ho; Liu, Hongxia; Qian, Guoliang; Liu, Fengquan.

In: Phytopathology, Vol. 106, No. 9, 09.2016, p. 971-977.

Research output: Contribution to journalArticle

Xu, Huiyong ; Chen, Hongfu ; Shen, Yuemao ; Du, Liangcheng ; Chou, Shan Ho ; Liu, Hongxia ; Qian, Guoliang ; Liu, Fengquan. / Direct regulation of extracellular chitinase production by the transcription factor LeClp in Lysobacter enzymogenes OH11. In: Phytopathology. 2016 ; Vol. 106, No. 9. pp. 971-977.
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AU - Liu, Hongxia

AU - Qian, Guoliang

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